TY - JOUR
T1 - Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria
AU - Law, Simon Sau Yin
AU - Liou, Geoffrey
AU - Nagai, Yukiko
AU - Giménez-Dejoz, Joan
AU - Tateishi, Ayaka
AU - Tsuchiya, Kousuke
AU - Kodama, Yutaka
AU - Fujigaya, Tsuyohiko
AU - Numata, Keiji
N1 - Funding Information:
We acknowledge the Support Unit for Bio-Material Analysis, RIKEN Center for Brain Science Research Resources Division, for performing the peptide syntheses and DNA sequencing and the Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, for the chlorophyll fluorescence experiments. We also acknowledge the RIKEN Information System Division for providing access to the Hokusai supercomputer for the molecular dynamics calculations. K.N. acknowledges the support from Japan Science and Technology Agency ERATO (Grant Number JPMJER1602), COI-NEXT, and the Grant-in-Aid for Transformative Research Areas (B) (Grant No. JP20H05735). T.F. thanks the support from Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering.
Funding Information:
We acknowledge the Support Unit for Bio-Material Analysis, RIKEN Center for Brain Science Research Resources Division, for performing the peptide syntheses and DNA sequencing and the Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, for the chlorophyll fluorescence experiments. We also acknowledge the RIKEN Information System Division for providing access to the Hokusai supercomputer for the molecular dynamics calculations. K.N. acknowledges the support from Japan Science and Technology Agency ERATO (Grant Number JPMJER1602), COI-NEXT, and the Grant-in-Aid for Transformative Research Areas (B) (Grant No. JP20H05735). T.F. thanks the support from Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - The delivery of genetic material into plants has been historically challenging due to the cell wall barrier, which blocks the passage of many biomolecules. Carbon nanotube-based delivery has emerged as a promising solution to this problem and has been shown to effectively deliver DNA and RNA into intact plants. Mitochondria are important targets due to their influence on agronomic traits, but delivery into this organelle has been limited to low efficiencies, restricting their potential in genetic engineering. This work describes the use of a carbon nanotube-polymer hybrid modified with functional peptides to deliver DNA into intact plant mitochondria with almost 30 times higher efficiency than existing methods. Genetic integration of a folate pathway gene in the mitochondria displays enhanced plant growth rates, suggesting its applications in metabolic engineering and the establishment of stable transformation in mitochondrial genomes. Furthermore, the flexibility of the polymer layer will also allow for the conjugation of other peptides and cargo targeting other organelles for broad applications in plant bioengineering.
AB - The delivery of genetic material into plants has been historically challenging due to the cell wall barrier, which blocks the passage of many biomolecules. Carbon nanotube-based delivery has emerged as a promising solution to this problem and has been shown to effectively deliver DNA and RNA into intact plants. Mitochondria are important targets due to their influence on agronomic traits, but delivery into this organelle has been limited to low efficiencies, restricting their potential in genetic engineering. This work describes the use of a carbon nanotube-polymer hybrid modified with functional peptides to deliver DNA into intact plant mitochondria with almost 30 times higher efficiency than existing methods. Genetic integration of a folate pathway gene in the mitochondria displays enhanced plant growth rates, suggesting its applications in metabolic engineering and the establishment of stable transformation in mitochondrial genomes. Furthermore, the flexibility of the polymer layer will also allow for the conjugation of other peptides and cargo targeting other organelles for broad applications in plant bioengineering.
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U2 - 10.1038/s41467-022-30185-y
DO - 10.1038/s41467-022-30185-y
M3 - Article
C2 - 35577779
AN - SCOPUS:85130132815
SN - 2041-1723
VL - 13
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 2417
ER -