TY - GEN
T1 - Poly (methyl methacrylate) microchip gel electrophoresis for the concentration and separation of aptamer and aptamer-thrombin complex with discontinuous buffers
AU - Wang, Jun
AU - Zhang, Yong
AU - Park, Yeon Su
AU - Okamoto, Yukihiro
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
PY - 2009
Y1 - 2009
N2 - A simple continuous isotachophoresis (ITP)-based microchip gel electrophoresis (MGE) was firstly applied for the concentration and separation of aptamer and its thrombin complex in a single cross-form poly (methyl methacrylate) (PMMA) microchip. The aptamer and its thrombin complex can be simultaneously concentrated and separated by this method which does not require time-consuming steps and complicated chip design. We found that different terminating electrolyte (TE) ions lead to different concentration enhancement factors and that lower mobility of TE ions can get higher concentration results. After optimization of various impact factors, we successfully achieved about 2000-fold concentration of aptamer-thrombin complex.
AB - A simple continuous isotachophoresis (ITP)-based microchip gel electrophoresis (MGE) was firstly applied for the concentration and separation of aptamer and its thrombin complex in a single cross-form poly (methyl methacrylate) (PMMA) microchip. The aptamer and its thrombin complex can be simultaneously concentrated and separated by this method which does not require time-consuming steps and complicated chip design. We found that different terminating electrolyte (TE) ions lead to different concentration enhancement factors and that lower mobility of TE ions can get higher concentration results. After optimization of various impact factors, we successfully achieved about 2000-fold concentration of aptamer-thrombin complex.
UR - http://www.scopus.com/inward/record.url?scp=84901814379&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901814379&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901814379
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 791
EP - 793
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -