TY - JOUR
T1 - Poly- and perfluoroalkyl substances in maternal serum
T2 - Method development and application in Pilot Study of the Japan Environment and Children's Study
AU - Nakayama, Shoji F.
AU - Isobe, Tomohiko
AU - Iwai-Shimada, Miyuki
AU - Kobayashi, Yayoi
AU - Nishihama, Yukiko
AU - Taniguchi, Yu
AU - Sekiyama, Makiko
AU - Michikawa, Takehiro
AU - Yamazaki, Shin
AU - Nitta, Hiroshi
AU - Oda, Masako
AU - Mitsubuchi, Hiroshi
AU - Sanefuji, Masafumi
AU - Ohga, Shouichi
AU - Mise, Nathan
AU - Ikegami, Akihiko
AU - Suga, Reiko
AU - Shimono, Masayuki
N1 - Funding Information:
We are grateful for the work of the staff of Pilot Study of the Japan Environment and Children's Study. The Japan Environment and Children's Study was funded by the Ministry of the Environment, Japan. The findings and conclusions of this article are solely the responsibility of the authors and do not represent the official views of the Japanese government.
Funding Information:
We are grateful for the work of the staff of Pilot Study of the Japan Environment and Children's Study. The Japan Environment and Children's Study was funded by the Ministry of the Environment , Japan. The findings and conclusions of this article are solely the responsibility of the authors and do not represent the official views of the Japanese government.
Publisher Copyright:
© 2020 The Author(s)
PY - 2020/5/10
Y1 - 2020/5/10
N2 - Poly- and perfluoroalkyl substances (PFAS) have been investigated in a number of cohort studies due to concern over their adverse health effects. The aim of this study was to develop a reliable, high throughput and cost-effective analytical method for a broad range of PFAS in human serum. Protein precipitation, automatic solid phase extraction (SPE) pre-treatment and column-switching LC-MS/MS were employed. The optimised and validated method was then used to analyse the levels of 28 PFAS in 339 maternal serum samples from Pilot Study of the Japan Environment and Children's Study. Perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluoroundecanoic acid (PFUnA) and perfluorooctane sulphonic acid (PFOS) were detected in all 339 samples at median (range) concentrations of 1.9 (0.46–15), 1.5 (0.32–10), 1.3 (0.25–4.5) and 3.7 (0.43–15) ng/ml, respectively. These levels are comparable to those reported in previous studies using samples collected from various parts of the world. With a few exceptions, the remainder of the PFAS examined had lower detection rates but were found at concentrations similar to those reported in previous studies. The sensitivity and throughput ability of the method developed here are sufficient for its application in a large-scale biomonitoring study.
AB - Poly- and perfluoroalkyl substances (PFAS) have been investigated in a number of cohort studies due to concern over their adverse health effects. The aim of this study was to develop a reliable, high throughput and cost-effective analytical method for a broad range of PFAS in human serum. Protein precipitation, automatic solid phase extraction (SPE) pre-treatment and column-switching LC-MS/MS were employed. The optimised and validated method was then used to analyse the levels of 28 PFAS in 339 maternal serum samples from Pilot Study of the Japan Environment and Children's Study. Perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluoroundecanoic acid (PFUnA) and perfluorooctane sulphonic acid (PFOS) were detected in all 339 samples at median (range) concentrations of 1.9 (0.46–15), 1.5 (0.32–10), 1.3 (0.25–4.5) and 3.7 (0.43–15) ng/ml, respectively. These levels are comparable to those reported in previous studies using samples collected from various parts of the world. With a few exceptions, the remainder of the PFAS examined had lower detection rates but were found at concentrations similar to those reported in previous studies. The sensitivity and throughput ability of the method developed here are sufficient for its application in a large-scale biomonitoring study.
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U2 - 10.1016/j.chroma.2020.460933
DO - 10.1016/j.chroma.2020.460933
M3 - Article
C2 - 32035665
AN - SCOPUS:85078930334
SN - 0021-9673
VL - 1618
JO - Journal of Chromatography A
JF - Journal of Chromatography A
M1 - 460933
ER -
