Polarity in archaeal operon transcription in Thermococcus kodakaraensis

Thomas J. Santangelo; L'ubomíra Čuboňová; Rie Matsumi; Haruyuki Atomi; Tadayuki Imanaka; John N. Reeve

doi:10.1128/JB.01811-07

Polarity in archaeal operon transcription in Thermococcus kodakaraensis

Thomas J. Santangelo, L'ubomíra Čuboňová, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, John N. Reeve

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.

Original language	English
Pages (from-to)	2244-2248
Number of pages	5
Journal	Journal of bacteriology
Volume	190
Issue number	6
DOIs	https://doi.org/10.1128/JB.01811-07
Publication status	Published - Mar 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology

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10.1128/JB.01811-07

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abstract = "An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.",

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AU - Santangelo, Thomas J.

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AU - Matsumi, Rie

AU - Atomi, Haruyuki

AU - Imanaka, Tadayuki

AU - Reeve, John N.

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N2 - An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.

AB - An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.

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Polarity in archaeal operon transcription in Thermococcus kodakaraensis

Abstract

All Science Journal Classification (ASJC) codes

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