TY - JOUR
T1 - PLLA/ZnO nanocomposites
T2 - Dynamic surfaces to harness cell differentiation
AU - Trujillo, Sara
AU - Lizundia, Erlantz
AU - Vilas, José Luis
AU - Salmeron-Sanchez, Manuel
N1 - Funding Information:
MSS acknowledges support from ERC through HealInSynergy ( 306990 ). EL thanks the University of the Basque Country (UPV/EHU) for a postdoctoral fellowship. ST acknowledges support from the University of Glasgow through their internal scholarship funding program . Technical and human support provided by SGIker (UPV/EHU, MICINN, GV/EJ, EGEF and ESF) is acknowledged. We gratefully acknowledge Corbion-Purac for the kind donation of PLLA.
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - This work investigates the effect of the sequential availability of ZnO nanoparticles, (nanorods of ~40 nm) loaded within a degradable poly(lactic acid) (PLLA) matrix, in cell differentiation. The system constitutes a dynamic surface, in which nanoparticles are exposed as the polymer matrix degrades. ZnO nanoparticles were loaded into PLLA and the system was measured at different time points to characterise the time evolution of the physicochemical properties, including wettability and thermal properties. The micro and nanostructure were also investigated using AFM, SEM and TEM images. Cellular experiments with C2C12 myoblasts show that cell differentiation was significantly enhanced on ZnO nanoparticles-loaded PLLA, as the polymer degrades and the availability of nanoparticles become more apparent, whereas the release of zinc within the culture medium was negligible. Our results suggest PLLA/ZnO nanocomposites can be used as a dynamic system where nanoparticles are exposed during degradation, activating the material surface and driving cell differentiation.
AB - This work investigates the effect of the sequential availability of ZnO nanoparticles, (nanorods of ~40 nm) loaded within a degradable poly(lactic acid) (PLLA) matrix, in cell differentiation. The system constitutes a dynamic surface, in which nanoparticles are exposed as the polymer matrix degrades. ZnO nanoparticles were loaded into PLLA and the system was measured at different time points to characterise the time evolution of the physicochemical properties, including wettability and thermal properties. The micro and nanostructure were also investigated using AFM, SEM and TEM images. Cellular experiments with C2C12 myoblasts show that cell differentiation was significantly enhanced on ZnO nanoparticles-loaded PLLA, as the polymer degrades and the availability of nanoparticles become more apparent, whereas the release of zinc within the culture medium was negligible. Our results suggest PLLA/ZnO nanocomposites can be used as a dynamic system where nanoparticles are exposed during degradation, activating the material surface and driving cell differentiation.
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U2 - 10.1016/j.colsurfb.2016.04.007
DO - 10.1016/j.colsurfb.2016.04.007
M3 - Article
C2 - 27085047
AN - SCOPUS:84962815079
SN - 0927-7765
VL - 144
SP - 152
EP - 160
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -