TY - JOUR
T1 - Plasmacytoid dendritic cells activate lymphoid-specific genetic programs irrespective of their cellular origin
AU - Shigematsu, Hirokazu
AU - Reizis, Boris
AU - Iwasaki, Hiromi
AU - Mizuno, Shin Ichi
AU - Hu, Dan
AU - Traver, David
AU - Leder, Philip
AU - Sakaguchi, Nobuo
AU - Akashi, Koichi
N1 - Funding Information:
We thank M.A. Handley for technical assistance with flow cytometric analysis and sorting, and M. Shinohara for the instruction for IFN-α measurement. This work was supported in part by grants from the NIH (DK050654, DK061320, and CA072009) and Damon-Runyon Cancer Research to K.A., and by the Uehara Memorial Foundation and Leukemia & Lymphoma Society to S.-i.M.
PY - 2004/7
Y1 - 2004/7
N2 - The developmental origin of type I interferon (IFN)-producing plasmacytoid dendritic cells (PDCs) is controversial. In particular, the rearrangement of immunoglobulin heavy chain (IgH) genes in murine PDCs and the expression of pre-T cell receptor α (pTα) gene by human PDCs were proposed as evidence for their "lymphoid" origin. Here we demonstrate that PDCs capable of IFN production develop efficiently from both myeloid- and lymphoid-committed progenitors. Rearranged IgH genes as well as RAG transcripts were found in both myeloid- and lymphoid-derived PDCs. The human pTα transgenic reporter was activated in both myeloid- and lymphoid-derived PDCs at a level comparable to pre-T cells. PDCs were the only cell population that activated murine RAG1 knockin and human pTα transgenic reporters outside the lymphoid lineage. These results highlight a unique developmental program of PDCs that distinguishes them from other cell types including conventional dendritic cells.
AB - The developmental origin of type I interferon (IFN)-producing plasmacytoid dendritic cells (PDCs) is controversial. In particular, the rearrangement of immunoglobulin heavy chain (IgH) genes in murine PDCs and the expression of pre-T cell receptor α (pTα) gene by human PDCs were proposed as evidence for their "lymphoid" origin. Here we demonstrate that PDCs capable of IFN production develop efficiently from both myeloid- and lymphoid-committed progenitors. Rearranged IgH genes as well as RAG transcripts were found in both myeloid- and lymphoid-derived PDCs. The human pTα transgenic reporter was activated in both myeloid- and lymphoid-derived PDCs at a level comparable to pre-T cells. PDCs were the only cell population that activated murine RAG1 knockin and human pTα transgenic reporters outside the lymphoid lineage. These results highlight a unique developmental program of PDCs that distinguishes them from other cell types including conventional dendritic cells.
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U2 - 10.1016/j.immuni.2004.06.011
DO - 10.1016/j.immuni.2004.06.011
M3 - Article
C2 - 15345219
AN - SCOPUS:3142749595
SN - 1074-7613
VL - 21
SP - 43
EP - 53
JO - Immunity
JF - Immunity
IS - 1
ER -
