TY - JOUR
T1 - Plasma treatment causes structural modifications in lysozyme, and increases cytotoxicity towards cancer cells
AU - Attri, Pankaj
AU - Kaushik, Nagendra Kumar
AU - Kaushik, Neha
AU - Hammerschmid, Dietmar
AU - Privat-Maldonado, Angela
AU - De Backer, Joey
AU - Shiratani, Masaharu
AU - Choi, Eun Ha
AU - Bogaerts, Annemie
N1 - Funding Information:
We gratefully acknowledge the European H2020 Marie Skłodowska-Curie Actions Individual Fellowship “Anticancer-PAM” within Horizon2020 (grant number 743546 ). This work was also supported by JSPS -KAKENHI grant number 20K14454 . NK thanks to National Research Foundation of Korea under Ministry of Science and ICT ( NRF-2021R1C1C1013875 ) of Korean Government. The computational work was carried out using the Turing HPC infrastructure at the CalcUA core facility of the Universiteit Antwerpen (UA), a division of the Flemish Supercomputer Center VSC, funded by the Hercules Foundation, the Flemish Government (department EWI) and the UA.
Funding Information:
We gratefully acknowledge the European H2020 Marie Sk?odowska-Curie Actions Individual Fellowship ?Anticancer-PAM? within Horizon2020 (grant number 743546). This work was also supported by JSPS-KAKENHI grant number 20K14454. NK thanks to National Research Foundation of Korea under Ministry of Science and ICT (NRF-2021R1C1C1013875) of Korean Government. The computational work was carried out using the Turing HPC infrastructure at the CalcUA core facility of the Universiteit Antwerpen (UA), a division of the Flemish Supercomputer Center VSC, funded by the Hercules Foundation, the Flemish Government (department EWI) and the UA.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/7/1
Y1 - 2021/7/1
N2 - Bacterial and mammalian proteins, such as lysozyme, are gaining increasing interest as anticancer drugs. This study aims to modify the lysozyme structure using cold atmospheric plasma to boost its cancer cell killing effect. We investigated the structure at acidic and neutral pH using various experimental techniques (circular dichroism, fluorescence, and mass spectrometry) and molecular dynamics simulations. The controlled structural modification of lysozyme at neutral pH enhances its activity, while the activity was lost at acidic pH at the same treatment conditions. Indeed, a larger number of amino acids were oxidized at acidic pH after plasma treatment, which results in a greater distortion of the lysozyme structure, whereas only limited structural changes were observed in lysozyme after plasma treatment at neutral pH. We found that the plasma-treated lysozyme significantly induced apoptosis to the cancer cells. Our results reveal that plasma-treated lysozyme could have potential as a new cancer cell killing drug.
AB - Bacterial and mammalian proteins, such as lysozyme, are gaining increasing interest as anticancer drugs. This study aims to modify the lysozyme structure using cold atmospheric plasma to boost its cancer cell killing effect. We investigated the structure at acidic and neutral pH using various experimental techniques (circular dichroism, fluorescence, and mass spectrometry) and molecular dynamics simulations. The controlled structural modification of lysozyme at neutral pH enhances its activity, while the activity was lost at acidic pH at the same treatment conditions. Indeed, a larger number of amino acids were oxidized at acidic pH after plasma treatment, which results in a greater distortion of the lysozyme structure, whereas only limited structural changes were observed in lysozyme after plasma treatment at neutral pH. We found that the plasma-treated lysozyme significantly induced apoptosis to the cancer cells. Our results reveal that plasma-treated lysozyme could have potential as a new cancer cell killing drug.
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U2 - 10.1016/j.ijbiomac.2021.05.146
DO - 10.1016/j.ijbiomac.2021.05.146
M3 - Article
C2 - 34051258
AN - SCOPUS:85107135379
SN - 0141-8130
VL - 182
SP - 1724
EP - 1736
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -
