TY - JOUR
T1 - Phylogeny and nitrogen fixation potential of Bradyrhizobium species isolated from the legume cover crop Pueraria phaseoloides (Roxb.) Benth. in Eastern Cameroon
AU - Sarr, Papa Saliou
AU - Araki, Shigeru
AU - Begoude, Didier Aime
AU - Yemefack, Martin
AU - Manga, Gabriel Ambroise
AU - Yamakawa, Takeo
AU - Htwe, Aung Zaw
N1 - Funding Information:
This work was supported by the SATREPS (Science and Technology Research Partnership for Sustainable Development) program (FOSAS: Cameroon Forest-Savanna Sustainability Project) of the Japan Science and Technology Agency (JST) and the Japan International Cooperation Agency (JICA), to which we express our sincere gratitude. We also would like to thank Mr. Dieudonne Ndong, the field research technician of the FOSAS project, for his valuable support during the field work. We also acknowledge Prof. Fumito Tani and Dr. Tetsuya Matsuda at the Laboratory of Food Environmental Science, Faculty of Agriculture, Kyoto University as well as Dr. Akifumi Sugiyama from the Research Institute for Sustainable Humanosphere (RISH) of Kyoto University for their collaboration during the molecular analysis.
Publisher Copyright:
© 2015 Japanese Society of Soil Science and Plant Nutrition.
PY - 2016/1/2
Y1 - 2016/1/2
N2 - Pueraria is an herbaceous, perennial legume crop originating in Asia. Pueraria phaseoloides (Roxb.) Benth. (tropical kudzu) is frequently introduced into production systems and is used as green manure, a cover crop and a forage plant, making it important economically. We used P. phaseoloides as a trap crop to study and characterize soil rhizobia in Eastern Cameroon. Bacteria were isolated from fresh nodules collected from field-grown P. phaseoloides roots. The 16S-23S rRNA internal transcribed spacer (ITS) sequences from 30 bacterial isolates were amplified by polymerase chain reaction (PCR) and the reaction products were sequenced. Phylogenetic analysis revealed that all isolates were ascribed to the genus Bradyrhizobium and were grouped into three clusters of Bradyrhizobium sp. strains, one cluster of B. yuanmingense strains, and one cluster of B. elkanii strains. Acetylene reduction assay (ARA) results indicated that the B. yuanmingense strains had significantly higher nitrogen fixation potential and that they could be used as inoculants to enhance nitrogen fixation in Pueraria grown in Eastern Cameroon.
AB - Pueraria is an herbaceous, perennial legume crop originating in Asia. Pueraria phaseoloides (Roxb.) Benth. (tropical kudzu) is frequently introduced into production systems and is used as green manure, a cover crop and a forage plant, making it important economically. We used P. phaseoloides as a trap crop to study and characterize soil rhizobia in Eastern Cameroon. Bacteria were isolated from fresh nodules collected from field-grown P. phaseoloides roots. The 16S-23S rRNA internal transcribed spacer (ITS) sequences from 30 bacterial isolates were amplified by polymerase chain reaction (PCR) and the reaction products were sequenced. Phylogenetic analysis revealed that all isolates were ascribed to the genus Bradyrhizobium and were grouped into three clusters of Bradyrhizobium sp. strains, one cluster of B. yuanmingense strains, and one cluster of B. elkanii strains. Acetylene reduction assay (ARA) results indicated that the B. yuanmingense strains had significantly higher nitrogen fixation potential and that they could be used as inoculants to enhance nitrogen fixation in Pueraria grown in Eastern Cameroon.
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U2 - 10.1080/00380768.2015.1086279
DO - 10.1080/00380768.2015.1086279
M3 - Article
AN - SCOPUS:84957851409
SN - 0038-0768
VL - 62
SP - 13
EP - 19
JO - Soil Science and Plant Nutrition
JF - Soil Science and Plant Nutrition
IS - 1
ER -