TY - JOUR
T1 - Phylogenetic analysis of soybean-nodulating rhizobia isolated from alkaline soils in Vietnam
AU - Saeki, Yuichi
AU - Kaneko, Ai
AU - Hara, Toshiaki
AU - Suzuki, Koutaro
AU - Yamakawa, Takeo
AU - Minh, Thi Nguyen
AU - Nagatomo, Yoshitaka
AU - Akao, Shoichiro
N1 - Funding Information:
Acknowledgments. This study was partially supported by a JICA project, The Education and Research Capability Building Project of Hanoi Agricultural University (HAU-JICA ERCB project, 1998-2003) and a Grant-in-Aid for Science Research (No.15780048). Japan Society for the Promotion of Science (JSPS).
PY - 2005/12
Y1 - 2005/12
N2 - In order to analyze the phylogeny of soybean-nodulating bacteria in alkaline soils in Vietnam, indigenous soybean-nodulating bacteria were isolated from root nodules by cultivating three kinds of Rj-soybean cultivars on two alkaline soils in Vietnam. The 120 isolates were classified into two major genera of soybean-nodulating rhizobia, namely Bradyrhizobium and Sinorhizobium genera, based on a growth analysis on medium and PCR-RFLP analyses of 16S rDNA and of the 16S-23S rDNA internal transcribed spacer (ITS) region. Most of the isolates of B. japonicum were extra-slow-growing and their ITS types were similar to that of B. japonicum USDA 135. They were not isolated from the soybean cultivar CNS used as Rj2Rj3 genotype. Isolates of Sinorhizobium were divided into two groups, S. fredii and S. sp., based on a PCR-RFLP analysis of 16S rDNA. Furthermore, PCR-RFLP analysis of the 16S-23S rDNA ITS region enabled to separate them into five types, three ITS types associated with S. fredii and two with S. sp. Sinorhizobium was frequently isolated from the three soybean cultivars on two soils. From the isolate ratio, it was suggested that B. japonicum strains similar to B. japonicum USDA 135 and S. fredii predominated in the alkaline soils of Vietnam. Additionally, our findings indicated that the Rj-genotypes affected not only the compatibility, but also the preference for nodulation between the host soybean and rhizobia.
AB - In order to analyze the phylogeny of soybean-nodulating bacteria in alkaline soils in Vietnam, indigenous soybean-nodulating bacteria were isolated from root nodules by cultivating three kinds of Rj-soybean cultivars on two alkaline soils in Vietnam. The 120 isolates were classified into two major genera of soybean-nodulating rhizobia, namely Bradyrhizobium and Sinorhizobium genera, based on a growth analysis on medium and PCR-RFLP analyses of 16S rDNA and of the 16S-23S rDNA internal transcribed spacer (ITS) region. Most of the isolates of B. japonicum were extra-slow-growing and their ITS types were similar to that of B. japonicum USDA 135. They were not isolated from the soybean cultivar CNS used as Rj2Rj3 genotype. Isolates of Sinorhizobium were divided into two groups, S. fredii and S. sp., based on a PCR-RFLP analysis of 16S rDNA. Furthermore, PCR-RFLP analysis of the 16S-23S rDNA ITS region enabled to separate them into five types, three ITS types associated with S. fredii and two with S. sp. Sinorhizobium was frequently isolated from the three soybean cultivars on two soils. From the isolate ratio, it was suggested that B. japonicum strains similar to B. japonicum USDA 135 and S. fredii predominated in the alkaline soils of Vietnam. Additionally, our findings indicated that the Rj-genotypes affected not only the compatibility, but also the preference for nodulation between the host soybean and rhizobia.
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U2 - 10.1111/j.1747-0765.2005.tb00143.x
DO - 10.1111/j.1747-0765.2005.tb00143.x
M3 - Article
AN - SCOPUS:30144435056
SN - 0038-0768
VL - 51
SP - 1043
EP - 1052
JO - Soil Science and Plant Nutrition
JF - Soil Science and Plant Nutrition
IS - 7
ER -