TY - JOUR
T1 - Phylogenetic analysis of Metarhizium spp. isolated from soil in Japan
AU - Nishi, Oumi
AU - Hasegawa, Keiichi
AU - Iiyama, Kazuhiro
AU - Yasunaga-Aoki, Chisa
AU - Shimizu, Susumu
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research no. 21380037 from the Japan Society for the Promotion of Science, which is promoted by the Ministry of Education, Culture, Sports, Science and Technology.
PY - 2011/8
Y1 - 2011/8
N2 - As a result of analyzing the internal transcribed spacer (ITS) and 5′ end of the EF-1α sequence of 145 isolates of Metarhizium spp. isolated from soil in Japan using selective agar medium, eight species were identified. ITS sequence analysis divided the isolates into three clades. Two were identified as M. flavoviride var. pemphigi and M. lepidiotae, respectively. EF-1α sequence analysis identified the other clades as six species: M. anisopliae, M. brunneum, M. guizhouense, M. majus, M. pingshaense and M. robertisii. The distribution of M. flavoviride var. pemphigi was restricted to forest or wood soil, and conidial sizes of M. guizhouense and M. majus were incongruent with the phylogeny based on the sequence of the 5′ end of EF-1α.
AB - As a result of analyzing the internal transcribed spacer (ITS) and 5′ end of the EF-1α sequence of 145 isolates of Metarhizium spp. isolated from soil in Japan using selective agar medium, eight species were identified. ITS sequence analysis divided the isolates into three clades. Two were identified as M. flavoviride var. pemphigi and M. lepidiotae, respectively. EF-1α sequence analysis identified the other clades as six species: M. anisopliae, M. brunneum, M. guizhouense, M. majus, M. pingshaense and M. robertisii. The distribution of M. flavoviride var. pemphigi was restricted to forest or wood soil, and conidial sizes of M. guizhouense and M. majus were incongruent with the phylogeny based on the sequence of the 5′ end of EF-1α.
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U2 - 10.1007/s13355-011-0045-y
DO - 10.1007/s13355-011-0045-y
M3 - Article
AN - SCOPUS:79961077041
SN - 0003-6862
VL - 46
SP - 301
EP - 309
JO - Applied Entomology and Zoology
JF - Applied Entomology and Zoology
IS - 3
ER -