Phylogenetic analysis of Metarhizium spp. isolated from soil in Japan

Oumi Nishi, Keiichi Hasegawa, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu

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29 Citations (Scopus)


As a result of analyzing the internal transcribed spacer (ITS) and 5′ end of the EF-1α sequence of 145 isolates of Metarhizium spp. isolated from soil in Japan using selective agar medium, eight species were identified. ITS sequence analysis divided the isolates into three clades. Two were identified as M. flavoviride var. pemphigi and M. lepidiotae, respectively. EF-1α sequence analysis identified the other clades as six species: M. anisopliae, M. brunneum, M. guizhouense, M. majus, M. pingshaense and M. robertisii. The distribution of M. flavoviride var. pemphigi was restricted to forest or wood soil, and conidial sizes of M. guizhouense and M. majus were incongruent with the phylogeny based on the sequence of the 5′ end of EF-1α.

Original languageEnglish
Pages (from-to)301-309
Number of pages9
JournalApplied Entomology and Zoology
Issue number3
Publication statusPublished - Aug 2011

All Science Journal Classification (ASJC) codes

  • Insect Science


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