TY - JOUR
T1 - Phospholipid-linked coumarin
T2 - A fluorescent probe for sensing hydroxyl radicals in lipid membranes
AU - Soh, Nobuaki
AU - Makihara, Koji
AU - Ariyoshi, Tomoyuki
AU - Seto, Daisuke
AU - Maki, Tomoharu
AU - Nakajima, Hizuru
AU - Nakano, Koji
AU - Imato, Toshihiko
PY - 2008/2
Y1 - 2008/2
N2 - A fluorescent probe, DPPEC (1,2-dipalmitoylglycerophosphorylethanolamine labeled with coumarin) was developed for detecting hydroxyl radical (•OH) in lipid membranes. The coumarin moiety contributes to the fluorescent detection of •OH and the phospholipids moiety gives a driving force to localize the probe in lipid membranes. DPPEC in liposomal membranes rapidly reacted with •OH and increased the fluorescence intensity, depending on the concentration of •OH. The increase in the fluorescence intensity induced by •OH was effectively suppressed by the addition of DMSO. The probe exhibited a higher fluorescence response to •OH over other reactive oxygen species, such as hydrogen peroxide, nitric oxide, peroxynitrite, alkylperoxyl radical, and hypochlorite. DPPEC would be useful as a new type of fluorescent probe that can localize in lipid membranes and detect •OH efficiently. 2008
AB - A fluorescent probe, DPPEC (1,2-dipalmitoylglycerophosphorylethanolamine labeled with coumarin) was developed for detecting hydroxyl radical (•OH) in lipid membranes. The coumarin moiety contributes to the fluorescent detection of •OH and the phospholipids moiety gives a driving force to localize the probe in lipid membranes. DPPEC in liposomal membranes rapidly reacted with •OH and increased the fluorescence intensity, depending on the concentration of •OH. The increase in the fluorescence intensity induced by •OH was effectively suppressed by the addition of DMSO. The probe exhibited a higher fluorescence response to •OH over other reactive oxygen species, such as hydrogen peroxide, nitric oxide, peroxynitrite, alkylperoxyl radical, and hypochlorite. DPPEC would be useful as a new type of fluorescent probe that can localize in lipid membranes and detect •OH efficiently. 2008
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U2 - 10.2116/analsci.24.293
DO - 10.2116/analsci.24.293
M3 - Article
C2 - 18270426
AN - SCOPUS:43049156448
SN - 0910-6340
VL - 24
SP - 293
EP - 296
JO - analytical sciences
JF - analytical sciences
IS - 2
ER -