@article{f69fa57d64254d9baca87821445ec545,
title = "Pharmacologic unmasking of epigenetically silenced tumor suppressor genes in esophageal squamous cell carcinoma",
abstract = "We performed a comprehensive survey of commonly inactivated tumor suppressor genes in esophageal squamous cell carcinoma (ESCC) based on functional reactivation of epigenetically silenced tumor suppressor genes by 5-aza-2′-deoxycytidine and trichostatin A using microarrays containing 12599 genes. Among 58 genes identified by this approach, 44 (76%) harbored dense CpG islands in the promoter regions. Thirteen of twenty-two tested gene promoters were methylated in cell lines, and ten in primary ESCC accompanied by silencing at the mRNA level. Potent growth suppressive activity of three genes including CRIP-1, Apolipoprotein D, and Neuromedin U in ESCC cells was demonstrated by colony focus assays. Pharmacologic reversal of epigenetic silencing is a powerful approach for comprehensive identification of tumor suppressor genes in human cancers.",
author = "Keishi Yamashita and Sunil Upadhyay and Motonobu Osada and Hoque, {Mohammad O.} and Yan Xiao and Masaki Mori and Fumiaki Sato and Meltzer, {Stephen J.} and David Sidransky",
note = "Funding Information: This work is supported by the NIH Grant U01CA84986-04 entitled, “Integrated Development of Novel Molecular Markers—The Early Detection Research Network: Biomarkers Developmental Laboratories” (EDRN Grant) and Grant U01CA85069-04 entitled, “Biomaker Development in Early Esophagogastric Cancer” (EDRN grant). Funding for the study described in this article was provided by Virco, Inc. Under a licensing agreement between The Johns Hopkins University and Virco, Dr. Sidransky is entitled to a share of royalty received by the University on sales or products described in this article. Dr. Sidransky is a paid consultant to Virco. The terms of this arrangement are being managed by The Johns Hopkins University in accordance with its conflict of interest policies. This work was also supported by The Medical Research Service, Department of Veterans.",
year = "2002",
month = dec,
doi = "10.1016/S1535-6108(02)00215-5",
language = "English",
volume = "2",
pages = "485--495",
journal = "Cancer Cell",
issn = "1535-6108",
publisher = "Cell Press",
number = "6",
}
