TY - JOUR
T1 - Pharmacokinetic study of ginsenosides Rb1 and Rg1 in rat by ELISA using anti-ginsenosides Rb1 and Rg1 monoclonal antibodies
AU - Chao, Zhi
AU - Shoyama, Yukihiro
AU - Tanaka, Hiroyuki
N1 - Funding Information:
This?work?was?supported?by?a?Grant-in-Aid?from?JSPS’s? Asia? Core?Program,?the?Ministry? of?Education,?Culture,?Sports,?Science?and?Technology? of?Japan,?a?Sasagawa?Medical? Scholarship,?a?Uehara?Memorial?Foundation,?a?research?fund?from?Kyushu?University? Foundation,?and?a?research?fund?from?Japan?Kampo?Medicine?Manufacturer.
PY - 2006
Y1 - 2006
N2 - Enzyme-linked immunosorbent assay (ELISA) systems using anti-ginsenoside Rb1 (G-Rb1) and Rg1 (G-Rg1) monoclonal antibodies (MAbs) were established for pharmacokinetic investigations of G-Rb1 and G-Rg1 in rat serum. The systems not only allowed sensitive detection of G-Rb1 at the level as low as 20 ng/ml and of G-Rg1 at 300 ng/ml, but showed strong capacity for detecting the two agents in a broad concentration range (20 to 400 ng/ml for G-Rb1 and 0.3 to 10 μg/ml for G-Rg1, respectively). In this respect, these assay systems are superior to other methods using thin-layer chromatography (TLC) or high-performance liquid chromatography (HPLC). In addition, another advantage of these immunoassays is the comparably low quantities of specimen required; as little as 5 μl of serum suffices the need for determination of ginsenosides. We report in this article the application of this immunoassay in pharmacokinetic study of G-Rb1.
AB - Enzyme-linked immunosorbent assay (ELISA) systems using anti-ginsenoside Rb1 (G-Rb1) and Rg1 (G-Rg1) monoclonal antibodies (MAbs) were established for pharmacokinetic investigations of G-Rb1 and G-Rg1 in rat serum. The systems not only allowed sensitive detection of G-Rb1 at the level as low as 20 ng/ml and of G-Rg1 at 300 ng/ml, but showed strong capacity for detecting the two agents in a broad concentration range (20 to 400 ng/ml for G-Rb1 and 0.3 to 10 μg/ml for G-Rg1, respectively). In this respect, these assay systems are superior to other methods using thin-layer chromatography (TLC) or high-performance liquid chromatography (HPLC). In addition, another advantage of these immunoassays is the comparably low quantities of specimen required; as little as 5 μl of serum suffices the need for determination of ginsenosides. We report in this article the application of this immunoassay in pharmacokinetic study of G-Rb1.
UR - http://www.scopus.com/inward/record.url?scp=33845533031&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845533031&partnerID=8YFLogxK
U2 - 10.1142/S0192415X06004533
DO - 10.1142/S0192415X06004533
M3 - Article
C2 - 17163595
AN - SCOPUS:33845533031
SN - 0192-415X
VL - 34
SP - 1069
EP - 1081
JO - American Journal of Chinese Medicine
JF - American Journal of Chinese Medicine
IS - 6
ER -