Pex11mediates peroxisomal proliferation by promoting deformation of the lipid membrane

Yumi Yoshida, Hajime Niwa, Masanori Honsho, Akinori Itoyama, Yukio Fujiki

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


Pex11p family proteins are key players in peroxisomal fission, but their molecular mechanisms remains mostly unknown. In the present study, overexpression of Pex11pb caused substantial vesiculation of peroxisomes in mammalian cells. This vesicle formation was dependent on dynamin-like protein 1 (DLP1) and mitochondrial fission factor (Mff), as knockdown of these proteins diminished peroxisomal fission after Pex11pb overexpression. The fission-deficient peroxisomes exhibited an elongated morphology, and peroxisomal marker proteins, such as Pex14p or matrix proteins harboring peroxisomal targeting signal 1, were discernible in a segmented staining pattern, like beads on a string. Endogenous Pex11pb was also distributed a striped pattern, but which was not coincide with Pex14p and PTS1 matrix proteins. Altered morphology of the lipid membrane was observed when recombinant Pex11p proteins were introduced into proteo-liposomes. Constriction of proteo-liposomes was observed under confocal microscopy and electron microscopy, and the reconstituted Pex11pb protein localized to the membrane constriction site. Introducing point mutations into the N-terminal amphiphathic helix of Pex11pb strongly reduced peroxisomal fission, and decreased the oligomer formation. These results suggest that Pex11p contributes to the morphogenesis of the peroxisomal membrane, which is required for subsequent fission by DLP1.

Original languageEnglish
Pages (from-to)710-721
Number of pages12
JournalBiology Open
Issue number6
Publication statusPublished - Jun 15 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)


Dive into the research topics of 'Pex11mediates peroxisomal proliferation by promoting deformation of the lipid membrane'. Together they form a unique fingerprint.

Cite this