TY - JOUR
T1 - Perylenebisimide-linked nitroxide for the detection of hydroxyl radicals
AU - Maki, Tomoharu
AU - Soh, Nobuaki
AU - Fukaminato, Tuyoshi
AU - Nakajima, Hizuru
AU - Nakano, Koji
AU - Imato, Toshihiko
N1 - Funding Information:
This work was supported by Industrial Technology Research Grant Program in 2005 (grant number; 05A01507a) from the New Energy and Industrial Technology Development Organization (NEDO) of Japan.
PY - 2009/4/20
Y1 - 2009/4/20
N2 - A new type of fluorophore-linked nitroxide for the detection of hydroxyl radical ({radical dot}OH) was synthesized using perylene-3,4,9,10-tetracarboxyl bisimide and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). This new fluorescent probe, indicated here as Spy-OH (swallow-tailed perylene derivative for detecting {radical dot}OH) has its natural fluorescence effectively reduced due to the quenching caused by the presence of the nitroxide radical in its structure. In the presence of {radical dot}OH, in media containing DMSO, a conspicuous increase in the fluorescence intensity was observed. No significant change in the fluorescence intensity was observed upon the addition of other several reactive oxygen species (ROS) except for {radical dot}OH. Spy-OH maxima excitation (λexc) and emission (λem) wavelengths lie in the visible region (520/530 nm), therefore, this fluorescent probe is expected to be useful for a simple and selective detection of {radical dot}OH, especially for biosamples.
AB - A new type of fluorophore-linked nitroxide for the detection of hydroxyl radical ({radical dot}OH) was synthesized using perylene-3,4,9,10-tetracarboxyl bisimide and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). This new fluorescent probe, indicated here as Spy-OH (swallow-tailed perylene derivative for detecting {radical dot}OH) has its natural fluorescence effectively reduced due to the quenching caused by the presence of the nitroxide radical in its structure. In the presence of {radical dot}OH, in media containing DMSO, a conspicuous increase in the fluorescence intensity was observed. No significant change in the fluorescence intensity was observed upon the addition of other several reactive oxygen species (ROS) except for {radical dot}OH. Spy-OH maxima excitation (λexc) and emission (λem) wavelengths lie in the visible region (520/530 nm), therefore, this fluorescent probe is expected to be useful for a simple and selective detection of {radical dot}OH, especially for biosamples.
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U2 - 10.1016/j.aca.2009.02.044
DO - 10.1016/j.aca.2009.02.044
M3 - Article
C2 - 19345762
AN - SCOPUS:63349098490
SN - 0003-2670
VL - 639
SP - 78
EP - 82
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1-2
ER -