TY - JOUR
T1 - Peroxiredoxin as a functional endogenous antioxidant enzyme in pronuclei of mouse zygotes
AU - Morita, Kohtaro
AU - Tokoro, Mikiko
AU - Hatanaka, Yuki
AU - Higuchi, Chika
AU - Ikegami, Haruka
AU - Nagai, Kouhei
AU - Anzai, Masayuki
AU - Kato, Hiromi
AU - Mitani, Tasuku
AU - Taguchi, Yoshitomo
AU - Yamagata, Kazuo
AU - Hosoi, Yoshihiko
AU - Miyamoto, Kei
AU - Matsumoto, Kazuya
N1 - Funding Information:
This study was supported, in part, by JSPS KAKENHI Grant Number JP25292189 (to KMa), a grant from the INAMORI Foundation (to KMa), the Human Frontier Science Program (RGP0021/2016) (to KMi), JSPS KAKENHI Grant Numbers JP16H01321, JP16H01222 and JP17H05045 (to KMi), and a Grant for Basic Science Research Projects from The Sumitomo Foundation (150810) (to KMi).
Funding Information:
We thank Ms N Backes-Kamimura and Mr J Horvat for English editing. This study was supported, in part, by JSPS KAKENHI Grant Number JP25292189 (to KMa), a grant from the INAMORI Foundation (to KMa), the Human Frontier Science Program (RGP0021/2016) (to KMi),JSPS KAKENHI Grant Numbers JP16H01321, JP16H01222 and JP17H05045 (to KMi), and a Grant for Basic Science Research Projects from The Sumitomo Foundation (150810) (to KMi).
Publisher Copyright:
© 2018 by the Society for Reproduction and Development.
PY - 2018
Y1 - 2018
N2 - Antioxidant mechanisms to adequately moderate levels of endogenous reactive oxygen species (ROS) are important for oocytes and embryos to obtain and maintain developmental competence, respectively. Immediately after fertilization, ROS levels in zygotes are elevated but the antioxidant mechanisms during the maternal-to-zygotic transition (MZT) are not well understood. First, we identified peroxiredoxin 1 (PRDX1) and PRDX2 by proteomics analysis as two of the most abundant endogenous antioxidant enzymes eliminating hydrogen peroxide (H2O2). We here report the cellular localization of hyperoxidized PRDX and its involvement in the antioxidant mechanisms of freshly fertilized oocytes. Treatment of zygotes at the pronuclear stage with H2O2 enhanced pronuclear localization of hyperoxidized PRDX in zygotes and concurrently impaired the generation of 5-hydroxymethylcytosine (5hmC) on the male genome, which is an epigenetic reprogramming event that occurs at the pronuclear stage. Thus, our results suggest that endogenous PRDX is involved in antioxidant mechanisms and epigenetic reprogramming during MZT.
AB - Antioxidant mechanisms to adequately moderate levels of endogenous reactive oxygen species (ROS) are important for oocytes and embryos to obtain and maintain developmental competence, respectively. Immediately after fertilization, ROS levels in zygotes are elevated but the antioxidant mechanisms during the maternal-to-zygotic transition (MZT) are not well understood. First, we identified peroxiredoxin 1 (PRDX1) and PRDX2 by proteomics analysis as two of the most abundant endogenous antioxidant enzymes eliminating hydrogen peroxide (H2O2). We here report the cellular localization of hyperoxidized PRDX and its involvement in the antioxidant mechanisms of freshly fertilized oocytes. Treatment of zygotes at the pronuclear stage with H2O2 enhanced pronuclear localization of hyperoxidized PRDX in zygotes and concurrently impaired the generation of 5-hydroxymethylcytosine (5hmC) on the male genome, which is an epigenetic reprogramming event that occurs at the pronuclear stage. Thus, our results suggest that endogenous PRDX is involved in antioxidant mechanisms and epigenetic reprogramming during MZT.
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U2 - 10.1262/jrd.2018-005
DO - 10.1262/jrd.2018-005
M3 - Article
C2 - 29503398
AN - SCOPUS:85045408985
SN - 0916-8818
VL - 64
SP - 161
EP - 171
JO - Journal of Reproduction and Development
JF - Journal of Reproduction and Development
IS - 2
ER -