TY - JOUR
T1 - Peroxidase activity and stability of surfactant-heme complex in nonaqueous media
AU - Kamiya, N.
AU - Furusaki, S.
AU - Goto, M.
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (No.09750841) from the Ministry of Education, Science, Sports and Culture of Japan, and by a foundation from The Association for the Progress of New Chemistry (ASPRONC). N.K. was supported by Research Fellowships of the Japan Society for the Promotion of Science (JSPS) for Young Scientists.
PY - 1997
Y1 - 1997
N2 - A surfactant-heme complex was prepared from hemin using a water-in-oil emulsion with a synthetic nonionic surfactant. The heme complex was soluble in anhydrous benzene with peroxidase activity for the oxidation of o-phenylene-diamine using tert-butyl hydroperoxide as an oxidant. An absorption spectrum of the heme complex in benzene was distinct from that of free heme in an aqueous buffer solution owing to the different aggregation states in the respective solution. Moreover, the heme complex could not be decomposed in benzene even in excess of the hydroperoxide due to enhanced stability.
AB - A surfactant-heme complex was prepared from hemin using a water-in-oil emulsion with a synthetic nonionic surfactant. The heme complex was soluble in anhydrous benzene with peroxidase activity for the oxidation of o-phenylene-diamine using tert-butyl hydroperoxide as an oxidant. An absorption spectrum of the heme complex in benzene was distinct from that of free heme in an aqueous buffer solution owing to the different aggregation states in the respective solution. Moreover, the heme complex could not be decomposed in benzene even in excess of the hydroperoxide due to enhanced stability.
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U2 - 10.1023/A:1018403518763
DO - 10.1023/A:1018403518763
M3 - Article
AN - SCOPUS:0030709315
SN - 0141-5492
VL - 19
SP - 1015
EP - 1018
JO - Biotechnology letters
JF - Biotechnology letters
IS - 10
ER -