TY - JOUR
T1 - Periostin in vitreoretinal diseases
AU - Yoshida, Shigeo
AU - Nakama, Takahito
AU - Ishikawa, Keijiro
AU - Nakao, Shintaro
AU - Sonoda, Koh hei
AU - Ishibashi, Tatsuro
N1 - Funding Information:
Acknowledgements We thank Drs. Kinuko Sasada, Yuki Kubo and Yoshiyuki Kobayashi for their fruitful discussions. We also thank Ms. Masayo Eto for her excellent technical assistance. This work was supported in part by JSPS KAKENHI Grant numbers 26293374, 26670757, 15H04995 and 16K15734.
Publisher Copyright:
© 2017, Springer International Publishing AG.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Proliferative vitreoretinal diseases such as diabetic retinopathy, proliferative vitreoretinopathy (PVR), and age-related macular degeneration are a leading cause of decreased vision and blindness in developed countries. In these diseases, retinal fibro(vascular) membrane (FVM) formation above and beneath the retina plays an important role. Gene expression profiling of human FVMs revealed significant upregulation of periostin. Subsequent analyses demonstrated increased periostin expression in the vitreous of patients with both proliferative diabetic retinopathy and PVR. Immunohistochemical analysis showed co-localization of periostin with α-SMA and M2 macrophage markers in FVMs. In vitro, periostin blockade inhibited migration and adhesion induced by PVR vitreous and transforming growth factor-β2 (TGF-β2). In vivo, a novel single-stranded RNAi agent targeting periostin showed the inhibitory effect on experimental retinal and choroidal FVM formation without affecting the viability of retinal cells. These results indicated that periostin is a pivotal molecule for FVM formation and a promising therapeutic target for these proliferative vitreoretinal diseases.
AB - Proliferative vitreoretinal diseases such as diabetic retinopathy, proliferative vitreoretinopathy (PVR), and age-related macular degeneration are a leading cause of decreased vision and blindness in developed countries. In these diseases, retinal fibro(vascular) membrane (FVM) formation above and beneath the retina plays an important role. Gene expression profiling of human FVMs revealed significant upregulation of periostin. Subsequent analyses demonstrated increased periostin expression in the vitreous of patients with both proliferative diabetic retinopathy and PVR. Immunohistochemical analysis showed co-localization of periostin with α-SMA and M2 macrophage markers in FVMs. In vitro, periostin blockade inhibited migration and adhesion induced by PVR vitreous and transforming growth factor-β2 (TGF-β2). In vivo, a novel single-stranded RNAi agent targeting periostin showed the inhibitory effect on experimental retinal and choroidal FVM formation without affecting the viability of retinal cells. These results indicated that periostin is a pivotal molecule for FVM formation and a promising therapeutic target for these proliferative vitreoretinal diseases.
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U2 - 10.1007/s00018-017-2651-5
DO - 10.1007/s00018-017-2651-5
M3 - Review article
C2 - 28913545
AN - SCOPUS:85029487134
SN - 1420-682X
VL - 74
SP - 4329
EP - 4337
JO - Cellular and Molecular Life Sciences
JF - Cellular and Molecular Life Sciences
IS - 23
ER -
