TY - GEN
T1 - Perfusion culture through a vasculature constructed in a tumor spheroid
AU - Nashimoto, Yuji
AU - Teraoka, Yukako
AU - Nakamasu, Akiko
AU - Hanada, Sanshiro
AU - Arima, Yuichiro
AU - Torisawa, Yu Suke
AU - Kotera, Hidetoshi
AU - Nishiyama, Koichi
AU - Miura, Takashi
AU - Yokokawa, Ryuji
N1 - Funding Information:
This work was supported by CREST, Japan Science and Technology Agency (JST), JSPS KAKENHI Grant Number 16K16386, Mizuho Foundation for the Promotion of Sciences. We also thank Yazaki Memorial Foundation for Science and Technology for supporting the travel.
Publisher Copyright:
© 17CBMS-0001.
PY - 2020
Y1 - 2020
N2 - We developed a microfluidic system for a perfusion culture of tumor spheroids utilizing engineered vasculatures. By the definition of the cellular interaction between fibroblast cells in a tumor spheroid and endothelial cells in microchannels, we successfully induced angiogenic sprouts from the microchannels, which anastomosed with the spheroid and formed perfusable vasculatures. We continuously supplied medium to the tumor spheroid through the vasculature and evaluated the effect of perfusion on the growth of the tumor spheroid. Our perfusion system greatly improved the cellular proliferation activity, mimicking the nutrition supply and evacuation of waste product through vasculatures surrounding tumors in vivo.
AB - We developed a microfluidic system for a perfusion culture of tumor spheroids utilizing engineered vasculatures. By the definition of the cellular interaction between fibroblast cells in a tumor spheroid and endothelial cells in microchannels, we successfully induced angiogenic sprouts from the microchannels, which anastomosed with the spheroid and formed perfusable vasculatures. We continuously supplied medium to the tumor spheroid through the vasculature and evaluated the effect of perfusion on the growth of the tumor spheroid. Our perfusion system greatly improved the cellular proliferation activity, mimicking the nutrition supply and evacuation of waste product through vasculatures surrounding tumors in vivo.
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M3 - Conference contribution
AN - SCOPUS:85079635582
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 1029
EP - 1030
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -