Peptide-Based Switching of Polymer Fluorescence in Aqueous phase

Hirotaka Ejima; Hiromi Kikuchi; Hisao Matsuno; Hirofumi Yajima; Takeshi Serizawa

doi:10.1021/cm102436s

Peptide-Based Switching of Polymer Fluorescence in Aqueous phase

Hirotaka Ejima, Hiromi Kikuchi, Hisao Matsuno, Hirofumi Yajima, Takeshi Serizawa

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The phage display method on water-soluble and commonly used poly-(2-methoxy-5-propyloxysulfonate-1,4-phenylenevinylene) (mpsPPV) were studied. mpsPPV was immobilized using a layer-by-layer (LbL) assembly technique with poly(diallyldimethylammonium chloride) (PDDA) to obtain water-insoluble polyelectrolyte multilayer films, with an outermost surface composed of mpsPPV, on a glass substrate. The assembly process and the stability of the obtained LbL films were monitored by quartz-crystal microbalance (QCM) measurement. A linear 12-mer peptide library solution was applied to this LbL film. The LbL film was considered to be stable throughout the phage display experiment, because the frequency shift was negligible after immersion in ethanol and Tris-buffered saline. It was suggested that π-π interactions were predominant between the peptide and mpsPPV.

Original language	English
Pages (from-to)	6032-6034
Number of pages	3
Journal	Chemistry of Materials
Volume	22
Issue number	22
DOIs	https://doi.org/10.1021/cm102436s
Publication status	Published - Nov 23 2010

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)
Materials Chemistry

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10.1021/cm102436s

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title = "Peptide-Based Switching of Polymer Fluorescence in Aqueous phase",

abstract = "The phage display method on water-soluble and commonly used poly-(2-methoxy-5-propyloxysulfonate-1,4-phenylenevinylene) (mpsPPV) were studied. mpsPPV was immobilized using a layer-by-layer (LbL) assembly technique with poly(diallyldimethylammonium chloride) (PDDA) to obtain water-insoluble polyelectrolyte multilayer films, with an outermost surface composed of mpsPPV, on a glass substrate. The assembly process and the stability of the obtained LbL films were monitored by quartz-crystal microbalance (QCM) measurement. A linear 12-mer peptide library solution was applied to this LbL film. The LbL film was considered to be stable throughout the phage display experiment, because the frequency shift was negligible after immersion in ethanol and Tris-buffered saline. It was suggested that π-π interactions were predominant between the peptide and mpsPPV.",

author = "Hirotaka Ejima and Hiromi Kikuchi and Hisao Matsuno and Hirofumi Yajima and Takeshi Serizawa",

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T1 - Peptide-Based Switching of Polymer Fluorescence in Aqueous phase

AU - Ejima, Hirotaka

AU - Kikuchi, Hiromi

AU - Matsuno, Hisao

AU - Yajima, Hirofumi

AU - Serizawa, Takeshi

PY - 2010/11/23

Y1 - 2010/11/23

N2 - The phage display method on water-soluble and commonly used poly-(2-methoxy-5-propyloxysulfonate-1,4-phenylenevinylene) (mpsPPV) were studied. mpsPPV was immobilized using a layer-by-layer (LbL) assembly technique with poly(diallyldimethylammonium chloride) (PDDA) to obtain water-insoluble polyelectrolyte multilayer films, with an outermost surface composed of mpsPPV, on a glass substrate. The assembly process and the stability of the obtained LbL films were monitored by quartz-crystal microbalance (QCM) measurement. A linear 12-mer peptide library solution was applied to this LbL film. The LbL film was considered to be stable throughout the phage display experiment, because the frequency shift was negligible after immersion in ethanol and Tris-buffered saline. It was suggested that π-π interactions were predominant between the peptide and mpsPPV.

AB - The phage display method on water-soluble and commonly used poly-(2-methoxy-5-propyloxysulfonate-1,4-phenylenevinylene) (mpsPPV) were studied. mpsPPV was immobilized using a layer-by-layer (LbL) assembly technique with poly(diallyldimethylammonium chloride) (PDDA) to obtain water-insoluble polyelectrolyte multilayer films, with an outermost surface composed of mpsPPV, on a glass substrate. The assembly process and the stability of the obtained LbL films were monitored by quartz-crystal microbalance (QCM) measurement. A linear 12-mer peptide library solution was applied to this LbL film. The LbL film was considered to be stable throughout the phage display experiment, because the frequency shift was negligible after immersion in ethanol and Tris-buffered saline. It was suggested that π-π interactions were predominant between the peptide and mpsPPV.

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ER -

Peptide-Based Switching of Polymer Fluorescence in Aqueous phase

Abstract

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