Peptide array for the comprehensive analysis of intracellular signals aiming to drug screening

Yoshiki Katayama; Tatsuhiko Sonoda; Yusuke Inoue; Takayuki Yamaji; Aishan Han; Kazuki Inamori; Osamu Okitsu; Masaharu Murata; Takuro Niidome

Peptide array for the comprehensive analysis of intracellular signals aiming to drug screening

Yoshiki Katayama, Tatsuhiko Sonoda, Yusuke Inoue, Takayuki Yamaji, Aishan Han, Kazuki Inamori, Osamu Okitsu, Masaharu Murata, Takuro Niidome

Center for Advanced Medical Open Innovation

Research output: Contribution to conference › Paper › peer-review

Abstract

New peptide array, on which many substrate peptides for protein kinases are immobilized, was developed. If the array was treated with isolated protein kinases or cell lysate, each peptide was phosphorylated dependent on their properties, after the optimized immobilization and masking procedures. Phosphorylation in each spot was detected by fluorescence on glass slide or 2 dimensional SPR on gold chip using anti-phospho antibody. In the glass array using Cy-3 labeled antibody for the detection, drug-specific profiles of peptide phosphorylation were obtained. Using this method, new assay system for the drug screening or fuctional analysis of unknown gene may be possible.

Original language	English
Pages	4849-4850
Number of pages	2
Publication status	Published - 2005
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: Sept 20 2005 → Sept 22 2005

Other

Other	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	9/20/05 → 9/22/05

All Science Journal Classification (ASJC) codes

Engineering(all)

Cite this

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title = "Peptide array for the comprehensive analysis of intracellular signals aiming to drug screening",

abstract = "New peptide array, on which many substrate peptides for protein kinases are immobilized, was developed. If the array was treated with isolated protein kinases or cell lysate, each peptide was phosphorylated dependent on their properties, after the optimized immobilization and masking procedures. Phosphorylation in each spot was detected by fluorescence on glass slide or 2 dimensional SPR on gold chip using anti-phospho antibody. In the glass array using Cy-3 labeled antibody for the detection, drug-specific profiles of peptide phosphorylation were obtained. Using this method, new assay system for the drug screening or fuctional analysis of unknown gene may be possible.",

author = "Yoshiki Katayama and Tatsuhiko Sonoda and Yusuke Inoue and Takayuki Yamaji and Aishan Han and Kazuki Inamori and Osamu Okitsu and Masaharu Murata and Takuro Niidome",

year = "2005",

language = "English",

pages = "4849--4850",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

}

TY - CONF

T1 - Peptide array for the comprehensive analysis of intracellular signals aiming to drug screening

AU - Katayama, Yoshiki

AU - Sonoda, Tatsuhiko

AU - Inoue, Yusuke

AU - Yamaji, Takayuki

AU - Han, Aishan

AU - Inamori, Kazuki

AU - Okitsu, Osamu

AU - Murata, Masaharu

AU - Niidome, Takuro

PY - 2005

Y1 - 2005

N2 - New peptide array, on which many substrate peptides for protein kinases are immobilized, was developed. If the array was treated with isolated protein kinases or cell lysate, each peptide was phosphorylated dependent on their properties, after the optimized immobilization and masking procedures. Phosphorylation in each spot was detected by fluorescence on glass slide or 2 dimensional SPR on gold chip using anti-phospho antibody. In the glass array using Cy-3 labeled antibody for the detection, drug-specific profiles of peptide phosphorylation were obtained. Using this method, new assay system for the drug screening or fuctional analysis of unknown gene may be possible.

AB - New peptide array, on which many substrate peptides for protein kinases are immobilized, was developed. If the array was treated with isolated protein kinases or cell lysate, each peptide was phosphorylated dependent on their properties, after the optimized immobilization and masking procedures. Phosphorylation in each spot was detected by fluorescence on glass slide or 2 dimensional SPR on gold chip using anti-phospho antibody. In the glass array using Cy-3 labeled antibody for the detection, drug-specific profiles of peptide phosphorylation were obtained. Using this method, new assay system for the drug screening or fuctional analysis of unknown gene may be possible.

UR - http://www.scopus.com/inward/record.url?scp=33645562991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645562991&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645562991

SP - 4849

EP - 4850

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Peptide array for the comprehensive analysis of intracellular signals aiming to drug screening

Abstract

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All Science Journal Classification (ASJC) codes

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