New peptide array, on which many substrate peptides for protein kinases are immobilized, was developed. If the array was treated with isolated protein kinases or cell lysate, each peptide was phosphorylated dependent on their properties, after the optimized immobilization and masking procedures. Phosphorylation in each spot was detected by fluorescence on glass slide or 2 dimensional SPR on gold chip using anti-phospho antibody. In the glass array using Cy-3 labeled antibody for the detection, drug-specific profiles of peptide phosphorylation were obtained. Using this method, new assay system for the drug screening or fuctional analysis of unknown gene may be possible.
|Number of pages||2|
|Publication status||Published - 2005|
|Event||54th SPSJ Symposium on Macromolecules - Yamagata, Japan|
Duration: Sept 20 2005 → Sept 22 2005
|Other||54th SPSJ Symposium on Macromolecules|
|Period||9/20/05 → 9/22/05|
All Science Journal Classification (ASJC) codes