TY - JOUR
T1 - Peg1/Mest in obese adipose tissue is expressed from the paternal allele in an isoform-specific manner
AU - Kamei, Yasutomi
AU - Suganami, Takayoshi
AU - Kohda, Takashi
AU - Ishino, Fumitoshi
AU - Yasuda, Kazuki
AU - Miura, Shinji
AU - Ezaki, Osamu
AU - Ogawa, Yoshihiro
N1 - Funding Information:
We thank A. Ito, M. Itoh, S. Kanai and A. Katsumata for technical assistance and the members of the Ogawa laboratory for helpful discussion. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and research grants from Takeda Science Foundation, Kanae Foundation for Life and Socio-Medical Science, and Mitsui Sumitomo Insurance Welfare Foundation. S.M. and O.E. were supported by the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation(NIBIO).
PY - 2007/1/9
Y1 - 2007/1/9
N2 - Paternally expressed 1 (Peg1)/mesoderm specific transcript (Mest) is an imprinted gene, which is only transcribed from the paternal (father's) allele. In some human cancer tissues, an alternatively spliced variant of PEG1/MEST mRNA using a different promoter of a distinct first exon is expressed from both paternal and maternal alleles. We previously reported that Peg1/Mest expression was markedly up-regulated in obese adipose tissue in mice. Moreover, transgenic overexpression of Peg1/Mest in the adipose tissue resulted in the enlargement of adipocytes in size. Given the potential pathophysiologic relevance in obesity, we examined the nature of increased expression of Peg1/Mest in obese adipose tissue. In obese adipose tissue, expression of Peg1/Mest was increased, but not that of other imprinted genes tested. The transcription rate of Peg1/Mest was increased in obese adipose tissue. We found at least four isoforms of mouse Peg1/Mest generated by use of the alternative first exons. We also demonstrated that the abundantly expressed Peg1/Mest in obese adipose tissue retained monoallelic expression. This is the first report of monoallelic induction of Peg1/Mest in adult tissues.
AB - Paternally expressed 1 (Peg1)/mesoderm specific transcript (Mest) is an imprinted gene, which is only transcribed from the paternal (father's) allele. In some human cancer tissues, an alternatively spliced variant of PEG1/MEST mRNA using a different promoter of a distinct first exon is expressed from both paternal and maternal alleles. We previously reported that Peg1/Mest expression was markedly up-regulated in obese adipose tissue in mice. Moreover, transgenic overexpression of Peg1/Mest in the adipose tissue resulted in the enlargement of adipocytes in size. Given the potential pathophysiologic relevance in obesity, we examined the nature of increased expression of Peg1/Mest in obese adipose tissue. In obese adipose tissue, expression of Peg1/Mest was increased, but not that of other imprinted genes tested. The transcription rate of Peg1/Mest was increased in obese adipose tissue. We found at least four isoforms of mouse Peg1/Mest generated by use of the alternative first exons. We also demonstrated that the abundantly expressed Peg1/Mest in obese adipose tissue retained monoallelic expression. This is the first report of monoallelic induction of Peg1/Mest in adult tissues.
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U2 - 10.1016/j.febslet.2006.12.002
DO - 10.1016/j.febslet.2006.12.002
M3 - Article
C2 - 17182038
AN - SCOPUS:33845662350
SN - 0014-5793
VL - 581
SP - 91
EP - 96
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -
