TY - JOUR
T1 - PDGFs regulate tooth germ proliferation and ameloblast differentiation
AU - Wu, Nan
AU - Iwamoto, Tsutomu
AU - Sugawara, Yu
AU - Futaki, Masaharu
AU - Yoshizaki, Keigo
AU - Yamamoto, Shinya
AU - Yamada, Aya
AU - Nakamura, Takashi
AU - Nonaka, Kazuaki
AU - Fukumoto, Satoshi
N1 - Funding Information:
This work was supported in part by grants-in-aid for Research Fellows of the Japan Society for the Promotion of Science from the Ministry of Education, Science and Culture of Japan ( 17689058 and 20679006 to S. Fukumoto).
PY - 2010/6
Y1 - 2010/6
N2 - Objective: The purpose of this study was to elucidate the effects of platelet-derived growth factors (PDGFs) during tooth development, as well as the mechanisms underlying the interactions of growth factors with PDGF signalling during odontogenesis. Design: We used an ex vivo tooth germ organ culture system and two dental cell lines, SF2 cells and mDP cells, as models of odontogenesis. AG17, a tyrosine kinase inhibitor, was utilised for blocking PDGF receptor signalling. To analyse the expressions of PDGFs, reverse transcriptase (RT)-PCR and immunohistochemistry were performed. Proliferation was examined using a BrdU incorporation assay for the organ cultures and a cell counting kit for the cell lines. The expressions of Fgf2 and ameloblastin were analysed by real-time RT-PCR. Results: The PDGF ligands PDGF-A and PDGF-B, and their receptors, PDGFRα and PDGFRβ, were expressed throughout the initial stages of tooth development. In the tooth germ organ cultures, PDGF-AA, but not PDGF-BB, accelerated cusp formation. Conversely, AG17 suppressed both growth and cusp formation of tooth germs. Exogenous PDGF-BB promoted mDP cell proliferation. Furthermore, PDGF-AA decreased Fgf2 expression and increased that of ameloblastin, a marker of differentiated ameloblasts. Conclusion: Our results indicate that PDGFs are involved in initial tooth development and regulate tooth size and shape, as well as ameloblast differentiation.
AB - Objective: The purpose of this study was to elucidate the effects of platelet-derived growth factors (PDGFs) during tooth development, as well as the mechanisms underlying the interactions of growth factors with PDGF signalling during odontogenesis. Design: We used an ex vivo tooth germ organ culture system and two dental cell lines, SF2 cells and mDP cells, as models of odontogenesis. AG17, a tyrosine kinase inhibitor, was utilised for blocking PDGF receptor signalling. To analyse the expressions of PDGFs, reverse transcriptase (RT)-PCR and immunohistochemistry were performed. Proliferation was examined using a BrdU incorporation assay for the organ cultures and a cell counting kit for the cell lines. The expressions of Fgf2 and ameloblastin were analysed by real-time RT-PCR. Results: The PDGF ligands PDGF-A and PDGF-B, and their receptors, PDGFRα and PDGFRβ, were expressed throughout the initial stages of tooth development. In the tooth germ organ cultures, PDGF-AA, but not PDGF-BB, accelerated cusp formation. Conversely, AG17 suppressed both growth and cusp formation of tooth germs. Exogenous PDGF-BB promoted mDP cell proliferation. Furthermore, PDGF-AA decreased Fgf2 expression and increased that of ameloblastin, a marker of differentiated ameloblasts. Conclusion: Our results indicate that PDGFs are involved in initial tooth development and regulate tooth size and shape, as well as ameloblast differentiation.
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U2 - 10.1016/j.archoralbio.2010.03.011
DO - 10.1016/j.archoralbio.2010.03.011
M3 - Article
C2 - 20392435
AN - SCOPUS:77955429105
SN - 0003-9969
VL - 55
SP - 426
EP - 434
JO - Archives of Oral Biology
JF - Archives of Oral Biology
IS - 6
ER -