TY - JOUR
T1 - PDGF-BB is a novel prognostic factor in colorectal cancer
AU - Nakamura, Yoshito
AU - Tanaka, Fumiaki
AU - Yoshikawa, Yasuji
AU - Mimori, Koshi
AU - Inoue, Hiroshi
AU - Yanaga, Katsuhiko
AU - Mori, Masaki
N1 - Funding Information:
We thank Dr. Koichi Tsuji for pathological assistance; we thank Drs. K. Ieta, N. Haraguchi, S. Hirasaki, Y. Kosaka, T. Yokoe, Z. Xiang, T. Ohmachi, K. Mimori, A. Sasaki, and H. Inoue for critical reading of the manuscript; and we also thank Ms. T. Shimooka, Ms. K. Ogata, Ms. M. Oda, Ms. N. Kasagi, and Ms. Y. Nakagawa for their excellent technical assistance. Financial support: This work was supported by the following grant sponsors: CREST, Japan Science and Technology Agency (JST); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research, grant numbers 17109013, 17591411, 17591413, 18390367, 18590333, 18659384, and 18790964; The Ministry of Education, Culture, Sports, Science, and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Areas, grant No. 18015039; Third Term Comprehensive Ten-Year Strategy for Cancer Control, grant No. 16271201.
Funding Information:
Grant sponsors: CREST, Japan Science and Technology Agency (JST); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research, grant numbers 17109013, 17591411, 17591413, 18390367, 18590333, 18659384, and 18790964; The Ministry of Education, Culture, Sports, Science, and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Areas, grant number 18015039; Third Term Comprehensive Ten-year Strategy for Cancer Control, grant number 16271201.
PY - 2008/8
Y1 - 2008/8
N2 - Purpose: Human platelet-derived growth factor BB (PDGF-BB) is thought to be involved in human malignancies. Its overexpression has been reported in some human tumors. However, its expression in colorectal cancer has not been studied. We thus investigated the clinicopathological and biological significance of PDGF-BB gene expression in human colorectal cancer. Experimental Design: Using real-time reverse transcription-PCR, we evaluated PDGF-BB expression status and correlated data with clinicopathological parameters in 60 patients with colorectal cancer. Additionally, we established a colorectal cancer cell line expressing PDGF-BB and investigated its effects on cell invasion and proliferation. Results: The incidence of vascular invasion was significantly greater in patients expressing PDGF-BB at a high level than in those at a low level (P < .05). Patients with high PDGF-BB expression had a significantly poorer survival rate than those with low PDGF-BB expression (P < .05). A multivariate analysis demonstrated that PDGF-BB expression was an independent prognostic factor. We demonstrated in vitro that cells transduced with PDGF-BB showed greater invasiveness (P < .05) and migration (P < .001) than did mock transduced cells. In a xenograft study, cells transduced with PDGF-BB had higher proliferation rates than mock transfected cells. Conclusion: PDGF-BB expression may be a new prognostic indicator for patients with colorectal cancer.
AB - Purpose: Human platelet-derived growth factor BB (PDGF-BB) is thought to be involved in human malignancies. Its overexpression has been reported in some human tumors. However, its expression in colorectal cancer has not been studied. We thus investigated the clinicopathological and biological significance of PDGF-BB gene expression in human colorectal cancer. Experimental Design: Using real-time reverse transcription-PCR, we evaluated PDGF-BB expression status and correlated data with clinicopathological parameters in 60 patients with colorectal cancer. Additionally, we established a colorectal cancer cell line expressing PDGF-BB and investigated its effects on cell invasion and proliferation. Results: The incidence of vascular invasion was significantly greater in patients expressing PDGF-BB at a high level than in those at a low level (P < .05). Patients with high PDGF-BB expression had a significantly poorer survival rate than those with low PDGF-BB expression (P < .05). A multivariate analysis demonstrated that PDGF-BB expression was an independent prognostic factor. We demonstrated in vitro that cells transduced with PDGF-BB showed greater invasiveness (P < .05) and migration (P < .001) than did mock transduced cells. In a xenograft study, cells transduced with PDGF-BB had higher proliferation rates than mock transfected cells. Conclusion: PDGF-BB expression may be a new prognostic indicator for patients with colorectal cancer.
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U2 - 10.1245/s10434-008-9943-9
DO - 10.1245/s10434-008-9943-9
M3 - Article
C2 - 18478301
AN - SCOPUS:48149096720
SN - 1068-9265
VL - 15
SP - 2129
EP - 2136
JO - Annals of Surgical Oncology
JF - Annals of Surgical Oncology
IS - 8
ER -
