TY - JOUR
T1 - Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori
AU - Chieda, Yuuka
AU - Iiyama, Kazuhiro
AU - Yasunaga-Aoki, Chisa
AU - Lee, Jae Man
AU - Kusakabe, Takahiro
AU - Shimizu, Susumu
PY - 2005/3/1
Y1 - 2005/3/1
N2 - To investigate the pathogenicity of Pseudomonas aeruginosa in insects, a gacA mutant of P. aeruginosa PA01 was constructed by site-directed mutagenesis. The mutant was designated as C1. C1 was less virulent to Bombyx mori than the parent strain. To complement the gacA gene, P. aeruginosa C1 was transformed with the broad host range plasmid pJB3Km1 carrying a 3.9-kbp gacA fragment. The expression of the gacA mRNA in C1 (pgacA) was detected. In addition, the complemented mutant restored the level and timing of pyocyanin production, indicating that functional GacA is produced in the complemented strain. However, no significant difference was observed between C1 and C1 (pgacA) with respect to the killing of B. mori larvae.
AB - To investigate the pathogenicity of Pseudomonas aeruginosa in insects, a gacA mutant of P. aeruginosa PA01 was constructed by site-directed mutagenesis. The mutant was designated as C1. C1 was less virulent to Bombyx mori than the parent strain. To complement the gacA gene, P. aeruginosa C1 was transformed with the broad host range plasmid pJB3Km1 carrying a 3.9-kbp gacA fragment. The expression of the gacA mRNA in C1 (pgacA) was detected. In addition, the complemented mutant restored the level and timing of pyocyanin production, indicating that functional GacA is produced in the complemented strain. However, no significant difference was observed between C1 and C1 (pgacA) with respect to the killing of B. mori larvae.
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U2 - 10.1016/j.femsle.2005.01.032
DO - 10.1016/j.femsle.2005.01.032
M3 - Article
C2 - 15727838
AN - SCOPUS:13844308534
SN - 0378-1097
VL - 244
SP - 181
EP - 186
JO - FEMS microbiology letters
JF - FEMS microbiology letters
IS - 1
ER -