Pathogenicity evaluation of Ralstonia pseudosolanacearum race 4 on ginger by leaf-clipping inoculation

Kazuhiro Iiyama; Momoka Imamura; Tomomi Inoue; Htet Wai Wai Kyaw; Kazutaka Yano; Mitsuo Horita; Kenichi Tsuchiya; Naruto Furuya

doi:10.1007/s10327-021-01017-6

Pathogenicity evaluation of Ralstonia pseudosolanacearum race 4 on ginger by leaf-clipping inoculation

Kazuhiro Iiyama, Momoka Imamura, Tomomi Inoue, Htet Wai Wai Kyaw, Kazutaka Yano, Mitsuo Horita, Kenichi Tsuchiya, Naruto Furuya

Agricultural Bioresource Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

An easy, rapid method has been needed to test the pathogenicity of strains of Ralstonia pseudosolanacearum, the cause of serious bacterial wilt on ginger (Zingiber officinale). When ginger leaves were clip-inoculated with race 4 strains, yellowing and V-shaped lesions developed on leaves, but no lesions formed after inoculation with race 1 strain or on mock-inoculated leaves. Tests of different bacterial concentrations as inoculum showed that the optimal concentration was greater than or equal to 10⁶ cfu/ml. This simple, high-throughput leaf clipping inoculation method provides an efficient, easy method to evaluate pathogenicity of R. pseudosolanacearum race 4 on ginger.

Original language	English
Pages (from-to)	269-272
Number of pages	4
Journal	Journal of General Plant Pathology
Volume	87
Issue number	5
DOIs	https://doi.org/10.1007/s10327-021-01017-6
Publication status	Published - Sept 2021

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Plant Science

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10.1007/s10327-021-01017-6

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title = "Pathogenicity evaluation of Ralstonia pseudosolanacearum race 4 on ginger by leaf-clipping inoculation",

abstract = "An easy, rapid method has been needed to test the pathogenicity of strains of Ralstonia pseudosolanacearum, the cause of serious bacterial wilt on ginger (Zingiber officinale). When ginger leaves were clip-inoculated with race 4 strains, yellowing and V-shaped lesions developed on leaves, but no lesions formed after inoculation with race 1 strain or on mock-inoculated leaves. Tests of different bacterial concentrations as inoculum showed that the optimal concentration was greater than or equal to 106 cfu/ml. This simple, high-throughput leaf clipping inoculation method provides an efficient, easy method to evaluate pathogenicity of R. pseudosolanacearum race 4 on ginger.",

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AU - Iiyama, Kazuhiro

AU - Imamura, Momoka

AU - Inoue, Tomomi

AU - Kyaw, Htet Wai Wai

AU - Yano, Kazutaka

AU - Horita, Mitsuo

AU - Tsuchiya, Kenichi

AU - Furuya, Naruto

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Pathogenicity evaluation of Ralstonia pseudosolanacearum race 4 on ginger by leaf-clipping inoculation

Abstract

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