TY - JOUR
T1 - Paraffin-embedded vertical sections of mouse embryonic stem cells
AU - Imai, Hiroyuki
AU - Fujii, Wataru
AU - Kusakabe, Ken Takeshi
AU - Kiso, Yasuo
AU - Ono, Etsuro
AU - Kano, Kiyoshi
N1 - Funding Information:
ACKNOWLEDGMENTS. This work was supported by the JSPS KAKENHI, Grant Numbers JP24658237, JP25660254, JP15K14880, and 17J07902, and the upgrade challenge project for JSPS KAKENHI Grant, Yamaguchi University.
Publisher Copyright:
©2018 The Japanese Society of Veterinary Science.
PY - 2018
Y1 - 2018
N2 - Cultured cells are generally observed through the bottom of dishes or flasks using an inverted microscope. Two-dimensional and horizontal observation is insufficient for histological analysis of several cell lines, such as embryonic stem cells or cancer cells, because they form three-dimensional colonies. In the present study, we aimed to establish a more informative method for analysis of such stereoscopic cultured cells. We cultured mouse embryonic stem cells using a temperature-sensitive culture dish, embedded these cells in paraffin, and successfully observed vertical sections of embryonic stem cells. This vertical analysis of the stereoscopic colony emphasized structural features such as the dome shape of naïve pluripotent stem cells. This method could have the potential for analysis of three-dimensional structures and histological preservation in cultured cells.
AB - Cultured cells are generally observed through the bottom of dishes or flasks using an inverted microscope. Two-dimensional and horizontal observation is insufficient for histological analysis of several cell lines, such as embryonic stem cells or cancer cells, because they form three-dimensional colonies. In the present study, we aimed to establish a more informative method for analysis of such stereoscopic cultured cells. We cultured mouse embryonic stem cells using a temperature-sensitive culture dish, embedded these cells in paraffin, and successfully observed vertical sections of embryonic stem cells. This vertical analysis of the stereoscopic colony emphasized structural features such as the dome shape of naïve pluripotent stem cells. This method could have the potential for analysis of three-dimensional structures and histological preservation in cultured cells.
UR - http://www.scopus.com/inward/record.url?scp=85054771525&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054771525&partnerID=8YFLogxK
U2 - 10.1292/jvms.18-0352
DO - 10.1292/jvms.18-0352
M3 - Comment/debate
C2 - 30089742
AN - SCOPUS:85054771525
SN - 0916-7250
VL - 80
SP - 1479
EP - 1481
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
IS - 10
ER -
