Pancreastatin molecular forms in normal human plasma

Kitayama Noboru Kitayama; Tateishi Kayoko Tateishi; Funakoshi Akihiro Funakoshi; Miyasak Kyoko Miyasak; Shimazoe Takao Shimazoe; Kono Akira Kono; Iwamoto Naoko Iwamoto; Matsuoka Yuji Matsuoka

doi:10.1016/0024-3205(94)90028-0

Pancreastatin molecular forms in normal human plasma

Kitayama Noboru Kitayama, Tateishi Kayoko Tateishi, Funakoshi Akihiro Funakoshi, Miyasak Kyoko Miyasak, Shimazoe Takao Shimazoe, Kono Akira Kono, Iwamoto Naoko Iwamoto, Matsuoka Yuji Matsuoka

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Circulating molecular forms with pancreastatin (PST)-like immunoreactivity in plasma from normal subjects were examined. An immunoreactive form corresponding to a human PST-like sequence [human chromogranin-A-(250-301)] (hPST-52) and a larger form (mol wt 15-21 kDa) were detected by gel filtration of plasma from normal subjects. On high performance liquid chromatography, predominant immunoreactive forms coeluted with the three larger forms which were purified from the xenograft of human pancreatic islet cell carcinoma cell line QGP-1N cells and with synthetic hPST-52. The fraction containing larger forms purified from xenograft of QGP-1N cells had biological activity equivalent to that of hPST-52 on the inhibition of pancreatic exocrine secretion. These results suggest that the larger molecular forms as well as hPST-52 may be physiologically important circulating forms of PST in human.

Original language	English
Pages (from-to)	1571-1578
Number of pages	8
Journal	Life Sciences
Volume	54
Issue number	21
DOIs	https://doi.org/10.1016/0024-3205(94)90028-0
Publication status	Published - 1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(94)90028-0

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title = "Pancreastatin molecular forms in normal human plasma",

abstract = "Circulating molecular forms with pancreastatin (PST)-like immunoreactivity in plasma from normal subjects were examined. An immunoreactive form corresponding to a human PST-like sequence [human chromogranin-A-(250-301)] (hPST-52) and a larger form (mol wt 15-21 kDa) were detected by gel filtration of plasma from normal subjects. On high performance liquid chromatography, predominant immunoreactive forms coeluted with the three larger forms which were purified from the xenograft of human pancreatic islet cell carcinoma cell line QGP-1N cells and with synthetic hPST-52. The fraction containing larger forms purified from xenograft of QGP-1N cells had biological activity equivalent to that of hPST-52 on the inhibition of pancreatic exocrine secretion. These results suggest that the larger molecular forms as well as hPST-52 may be physiologically important circulating forms of PST in human.",

author = "{Noboru Kitayama}, Kitayama and {Kayoko Tateishi}, Tateishi and {Akihiro Funakoshi}, Funakoshi and {Kyoko Miyasak}, Miyasak and {Takao Shimazoe}, Shimazoe and {Akira Kono}, Kono and {Naoko Iwamoto}, Iwamoto and {Yuji Matsuoka}, Matsuoka",

year = "1994",

doi = "10.1016/0024-3205(94)90028-0",

language = "English",

volume = "54",

pages = "1571--1578",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "21",

}

TY - JOUR

T1 - Pancreastatin molecular forms in normal human plasma

AU - Noboru Kitayama, Kitayama

AU - Kayoko Tateishi, Tateishi

AU - Akihiro Funakoshi, Funakoshi

AU - Kyoko Miyasak, Miyasak

AU - Takao Shimazoe, Shimazoe

AU - Akira Kono, Kono

AU - Naoko Iwamoto, Iwamoto

AU - Yuji Matsuoka, Matsuoka

PY - 1994

Y1 - 1994

N2 - Circulating molecular forms with pancreastatin (PST)-like immunoreactivity in plasma from normal subjects were examined. An immunoreactive form corresponding to a human PST-like sequence [human chromogranin-A-(250-301)] (hPST-52) and a larger form (mol wt 15-21 kDa) were detected by gel filtration of plasma from normal subjects. On high performance liquid chromatography, predominant immunoreactive forms coeluted with the three larger forms which were purified from the xenograft of human pancreatic islet cell carcinoma cell line QGP-1N cells and with synthetic hPST-52. The fraction containing larger forms purified from xenograft of QGP-1N cells had biological activity equivalent to that of hPST-52 on the inhibition of pancreatic exocrine secretion. These results suggest that the larger molecular forms as well as hPST-52 may be physiologically important circulating forms of PST in human.

AB - Circulating molecular forms with pancreastatin (PST)-like immunoreactivity in plasma from normal subjects were examined. An immunoreactive form corresponding to a human PST-like sequence [human chromogranin-A-(250-301)] (hPST-52) and a larger form (mol wt 15-21 kDa) were detected by gel filtration of plasma from normal subjects. On high performance liquid chromatography, predominant immunoreactive forms coeluted with the three larger forms which were purified from the xenograft of human pancreatic islet cell carcinoma cell line QGP-1N cells and with synthetic hPST-52. The fraction containing larger forms purified from xenograft of QGP-1N cells had biological activity equivalent to that of hPST-52 on the inhibition of pancreatic exocrine secretion. These results suggest that the larger molecular forms as well as hPST-52 may be physiologically important circulating forms of PST in human.

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DO - 10.1016/0024-3205(94)90028-0

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VL - 54

SP - 1571

EP - 1578

JO - Life Sciences

JF - Life Sciences

IS - 21

ER -

Pancreastatin molecular forms in normal human plasma

Abstract

All Science Journal Classification (ASJC) codes

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