p53 status-dependent sensitization of human tumour cells to hyperthermia by plant flavonol

Tomoyuki Hamamoto, Keiji Suzuki, Motohiro Yamauchi, Seiji Kodama, Hitoshi Sasaki, Masami Watanabe

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Purpose: Quercetin (QCT), an important flavonol, is known to sensitize tumour cells to hyperthermia by suppressing heat shock protein 72 (Hsp72) induction, and is also reported to inhibit p53 accumulation. This study was conducted to examine the effects of QCT on the heat sensitivities of human tumour cell lines with different p53 statuses. Material and methods: Cell lines derived from human cancers and p53-inducible cells were used. After heat treatment at 43°C for 2 h with or without QCT, cell survival was determined in a clonogenic assay. The cellular and nuclear content of Hsp72 as well as that of p53 was determined by Western blotting analysis. Results: Treatment of cells with 150 μM QCT, which completely abolished Hsp72 induction, potentiated the lethal effects of hyperthermia in all tumour cell lines. Particularly, remarkable enhancement of cell death was observed in tumour cell lines having little or no p53 proteins. Although nuclear translocation of Hsp72 is induced by hyperthermia, it was significantly compromised in p53-deficient cells. Conclusions: These results indicate that p53 is a component for nuclear accumulation of Hsp72; therefore, p53 status is an important determinant of the sensitization of human tumour cells to hyperthermia by QCT.

Original languageEnglish
Pages (from-to)415-424
Number of pages10
JournalInternational Journal of Hyperthermia
Issue number5
Publication statusPublished - 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)
  • Cancer Research


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