TY - JOUR
T1 - Overall interaction of cytosolic proteins with the PEI/DNA complex
AU - Iida, Takayuki
AU - Mori, Takeshi
AU - Katayama, Yoshiki
AU - Niidome, Takuro
N1 - Funding Information:
The authors thank Dr. Sannamu Lee and Dr. Masanobu Desimaru in the Faculty of Science, Fukuoka University for their technical support for MALDI-TOF-MS measurements of the peptide fragment. This research was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (No. 16790106), and a Grant-in-Aid for Scientific Research in the Priority Area “Molecular Nano Dynamics” (No. 17034049) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2007/4/23
Y1 - 2007/4/23
N2 - Little is known on mechanisms involved in transport of complex of DNA and gene carrier molecules from the cytosol to the nucleus. We aimed to identify cytosolic proteins interacting with the polyethylenimine (PEI)/DNA complex, using 2-D gel electrophoresis and peptide mass fingerprinting. Fifteen proteins including actin, beta-tubulin, and other metabolic proteins were identified. They demonstrated various molecular weights and isoelectric points, and were categorized into 3 groups: early binding, late binding, and transient binding proteins. Protein binding caused DNA release from the PEI/DNA complex with a cation/anion (C/A) ratio of 2, where complex formation was weak. Knowledge on interactions between cytosolic proteins and DNA/carrier complexes will help understand intracellular gene delivery mechanisms.
AB - Little is known on mechanisms involved in transport of complex of DNA and gene carrier molecules from the cytosol to the nucleus. We aimed to identify cytosolic proteins interacting with the polyethylenimine (PEI)/DNA complex, using 2-D gel electrophoresis and peptide mass fingerprinting. Fifteen proteins including actin, beta-tubulin, and other metabolic proteins were identified. They demonstrated various molecular weights and isoelectric points, and were categorized into 3 groups: early binding, late binding, and transient binding proteins. Protein binding caused DNA release from the PEI/DNA complex with a cation/anion (C/A) ratio of 2, where complex formation was weak. Knowledge on interactions between cytosolic proteins and DNA/carrier complexes will help understand intracellular gene delivery mechanisms.
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U2 - 10.1016/j.jconrel.2006.12.027
DO - 10.1016/j.jconrel.2006.12.027
M3 - Article
C2 - 17292504
AN - SCOPUS:33947326434
SN - 0168-3659
VL - 118
SP - 364
EP - 369
JO - Journal of Controlled Release
JF - Journal of Controlled Release
IS - 3
ER -