TY - GEN
T1 - Over 50,000-fold sample preconcentration efficiency in microchip electrophoresis using a simple channel
AU - Kawai, T.
AU - Ueda, M.
AU - Sueyoshi, K.
AU - Kitagawa, F.
AU - Otsuka, K.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - Combination of two online sample preconcentration techniques, large-volume sample stacking with an electroosmotic flow pump (LVSEP) and field-amplified sample injection (FASI), was investigated to improve a poor sensitivity of microchip electrophoresis. In the LVSEP-FASI-microchip zone electrophoresis (MCZE) analysis of fluorescein, a 60,000-fold sensitivity increase was achieved both in the single straight channel and Y-channel compared with conventional MCZE. A baseline separation of maltoheptaose (G7) and free dye was also obtained with a 10,000-fold sensitivity increase by LVSEP-FASI in the capillary-based single channel analysis. The relative standard deviations of the detection time and the peak height were 2.4% and 11%, respectively.
AB - Combination of two online sample preconcentration techniques, large-volume sample stacking with an electroosmotic flow pump (LVSEP) and field-amplified sample injection (FASI), was investigated to improve a poor sensitivity of microchip electrophoresis. In the LVSEP-FASI-microchip zone electrophoresis (MCZE) analysis of fluorescein, a 60,000-fold sensitivity increase was achieved both in the single straight channel and Y-channel compared with conventional MCZE. A baseline separation of maltoheptaose (G7) and free dye was also obtained with a 10,000-fold sensitivity increase by LVSEP-FASI in the capillary-based single channel analysis. The relative standard deviations of the detection time and the peak height were 2.4% and 11%, respectively.
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M3 - Conference contribution
AN - SCOPUS:84883755430
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 2022
EP - 2024
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -