Osteoclast behaviors on the surface of deproteinized bovine bone mineral and carbonate apatite substitutes in vitro

Masako Fujioka-Kobayashi, Youji Miyamoto, Kunio Ishikawa, Takafumi Satomi, Benoit Schaller

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


The present study investigated the osteoclast differentiation potential and paracrine effects of osteoclasts on osteoblast differentiation when the cells were cultured directly on two bone substitutes (BSs): deproteinized bovine bone mineral (DBBM) and carbonate apatite (CO3Ap). Human primary osteoclasts cultured on the BSs were assessed by tartrate-resistant acid phosphatase (TRAP) and actin ring staining. Thereafter, the mRNA levels of osteoclastic differentiation markers were quantified by real-time PCR. Osteoblast behaviors in response to conditioned media collected from osteoclast cultures were investigated. Interestingly, mature osteoclasts were occasionally observed on the surface of the CO3Ap granules, whereas very few and small osteoclasts were observed on DBBM. Similarly, real-time PCR analysis showed higher mRNA levels of osteoclast markers, including cathepsin K and TRAP, in the cells cultured on CO3Ap than in those cultured on DBBM. Furthermore, compared to DBBM, CO3Ap promoted osteoblast differentiation in human primary osteoblasts, whereas few paracrine effects of osteoclasts cultured with either BS were observed on the osteoblast differentiation potential. These limited results showed that CO3Ap provided a favorable surface for osteoclast differentiation, as well as osteoblasts, compared to DBBM in vitro.

Original languageEnglish
Pages (from-to)1524-1532
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Issue number8
Publication statusPublished - Aug 2022

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys


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