Oscillatory membrane currents paradoxically induced via NO-activated pathways in detrusor cells

Shunichi Kajioka, Shinsuke Nakayama, Narihito Seki, Seiji Naito, Alison F. Brading

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9 Citations (Scopus)


Oscillatory inward membrane currents (Ioscil-in) reflecting intracellular Ca2+ ([Ca2+]i) activity in detrusor cells, are thought to play an important role in producing tonic bladder contractions during micturition. The present patch clamp study revealed a new activation mechanism: sodium nitroprusside (SNP), a nitric oxide (NO) donor induced Ioscil-in in a subpopulation of detrusor cells. The inhibitory effect of niflumic acid on SNP-induced Ioscil-in suggests that Ca2+-activated Cl- channels are responsible for this current. In addition, SNP-induced Ioscil-in required the cooperation of Ca2+ influx through SK&F96365-sensitive channels and intracellular Ca2+ release channels sensitive to ryanodine but insensitive to xestospongin C (XeC). This is also true for muscarinic agonist (carbachol: CCh)-induced Ioscil-in. However, 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a guanylyl cyclase inhibitor, suppressed SNP-induced Ioscil-in but not CCh-induced Ioscil-in. The results suggest that a subpopulation of detrusor cells employ the NO/cGMP cascade to potentiate bladder contraction. Mechanisms underlying NO-induced Ioscil-in are likely to contribute not only to the physiology but also to the pathophysiology of the lower urinary tract.

Original languageEnglish
Pages (from-to)202-209
Number of pages8
JournalCell Calcium
Issue number2
Publication statusPublished - Aug 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology


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