Organization, structure, chromosomal assignment, and expression of the gene encoding the human endothelin-A receptor

Kiminori Hosoda, Kazuwa Nakao, Naohisa Tamura, Hiroshi Arai, Yoshihiro Ogawa, Shin Ichi Suga, Shigetada Nakanishi, Hiroo Imura

Research output: Contribution to journalArticlepeer-review

168 Citations (Scopus)

Abstract

We have isolated and characterized the gene for the human endothelin-A receptor. Southern blot analyses demonstrated a single copy gene for the receptor. The gene spans more than 40 kilobases and contains eight exons and seven introns. Intron 1 exists in the 5′-noncoding region, and introns 2-7 occur in the coding region. The locations of introns 2-7 exist before or after the regions encoding the membrane-spanning domains. The transcription start site, determined by primer extension experiments, is 502 base pairs upstream of the methionine initiation codon. The 5′-flanking region lacks a typical TATA box but contains a potential SP-1-binding site 27 base pairs upstream of the transcription start site. Using human-rodent somatic hybrid cell DNA, the gene was assigned to human chromosome 4. Northern blot analyses revealed a 4.3-kilobase mRNA in a wide variety of human tissues, at the highest level in the aorta and at a substantial level in the cultured human mesangial cells. This is the first report of cloning of a gene for a member of the endothelin receptor family. The present study should give a clue to the discovery of possible disorders of the endothelin-A receptor, as well as facilitate the elucidation of the mechanisms by which the gene expression is regulated.

Original languageEnglish
Pages (from-to)18797-18804
Number of pages8
JournalJournal of Biological Chemistry
Volume267
Issue number26
Publication statusPublished - Sept 15 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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