TY - JOUR
T1 - Optical coherence tomography-based tissue dynamics imaging for longitudinal and drug response evaluation of tumor spheroids
AU - El-Sadek, IBrahim Abd
AU - Miyazawa, Arata
AU - Shen, Larina Tzu Wei
AU - Makita, Shuichi
AU - Fukuda, Shinichi
AU - Yamashita, Toshiharu
AU - Oka, Yuki
AU - Mukherjee, Pradipta
AU - Matsusaka, Satoshi
AU - Oshika, Tetsuro
AU - Kano, Hideaki
AU - Yasuno, Yoshiaki
N1 - Funding Information:
Japan Society for the Promotion of Science (15K13371, 18H01893, 18J13841, 18K19961); Japan Science and Technology Agency (JPMJMI18G8).
Funding Information:
Although this research was funded solely by the agency listed in the funding section, the project is tightly related to a joint research project between Yokogawa Electric Corp. and the University of Tsukuba. The authors greatly appreciate fruitful technical discussions held with Hiroyuki Sangu (Yokogawa), Atsushi Kubota and Renzo Ikeda (Skytechnology), Akihiro Shitoh and Yuichi Inoue (Optosigma), Masato Takaya (Tatsuta), and Naoki Fukutake (Nikon) are highly appreciated. Hideaki Kano is currently at Department of Chemistry, Faculty of Science, Kyushu University.
Publisher Copyright:
© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - We present optical coherence tomography (OCT)-based tissue dynamics imaging method to visualize and quantify tissue dynamics such as subcellular motion based on statistical analysis of rapid-time-sequence OCT signals at the same location. The analyses include logarithmic intensity variance (LIV) method and two types of OCT correlation decay speed analysis (OCDS). LIV is sensitive to the magnitude of the signal fluctuations, while OCDSs including early- and late-OCDS (OCDSe and OCDSl, respectively) are sensitive to the fast and slow tissue dynamics, respectively. These methods were able to visualize and quantify the longitudinal necrotic process of a human breast adenocarcinoma spheroid and its anti-cancer drug response. Additionally, the effects of the number of OCT signals and the total acquisition time on dynamics imaging are examined. Small number of OCT signals, e.g., five or nine suffice for dynamics imaging when the total acquisition time is suitably long.
AB - We present optical coherence tomography (OCT)-based tissue dynamics imaging method to visualize and quantify tissue dynamics such as subcellular motion based on statistical analysis of rapid-time-sequence OCT signals at the same location. The analyses include logarithmic intensity variance (LIV) method and two types of OCT correlation decay speed analysis (OCDS). LIV is sensitive to the magnitude of the signal fluctuations, while OCDSs including early- and late-OCDS (OCDSe and OCDSl, respectively) are sensitive to the fast and slow tissue dynamics, respectively. These methods were able to visualize and quantify the longitudinal necrotic process of a human breast adenocarcinoma spheroid and its anti-cancer drug response. Additionally, the effects of the number of OCT signals and the total acquisition time on dynamics imaging are examined. Small number of OCT signals, e.g., five or nine suffice for dynamics imaging when the total acquisition time is suitably long.
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U2 - 10.1364/BOE.404336
DO - 10.1364/BOE.404336
M3 - Article
AN - SCOPUS:85094894997
SN - 2156-7085
VL - 11
SP - 6231
EP - 6248
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 11
ER -
