Optical characterization of the antigen-antibody thin layer using the whispering gallery mode

Takeshi Tajiri, Shuzo Matsumoto, Toshihiko Imato, Toshihiro Okamoto, Masanobu Haraguchi

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    We immobilized an antibody (anti-β-Galactosidase) on a polystyrene microsphere by using a covalent bond, and observed the resonance peaks in the scattered light intensity spectra related to the whispering gallery mode (WGM) excitation of the microsphere. The amount and the optical parameters, i.e., thickness and refractive index, of anti-β-Galactosidase on the sphere surface were evaluated based on an absorbance measurement and a resonance peak shift measurement, respectively. Moreover, we measured the variation of the WGM spectra depending on the concentration of the enzyme solution (β-Galactosidase), which allowed us to optically evaluate the thickness and the refractive index of the antigen-antibody layer from the shift of the WGM spectra peak.

    Original languageEnglish
    Pages (from-to)799-804
    Number of pages6
    Journalanalytical sciences
    Volume30
    Issue number8
    DOIs
    Publication statusPublished - 2014

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry

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