TY - JOUR
T1 - Opsonic effect of the third complement component (C3) of carp (Cyprinus carpio) on phagocytosis by neutrophils
AU - Matsuyama, H.
AU - Yano, T.
AU - Yamakawa, T.
AU - Nakao, M.
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (C) and by a Grant-in-Aid for Encouragement of Young Scientists from the Ministry of Education, Science and Culture, Japan.
PY - 1992/1
Y1 - 1992/1
N2 - The opsonic effect of carp complement on the phagocytosis by carp peritoneal neutrophils was investigated using antibody-sensitised sheep erythrocytes (EA) and baker's yeast (Y), which are the activators of the classical complement pathway (CCP) and the alternative complement pathway (ACP), respectively. Carp antibody (A) exerted little opsonic effect on the phagocytosis of sheep erythrocytes (E). The treatment of EA and Y with normal carp serum (C) greatly enhanced phagocytosis. Such opsonic effect is apparently due to the complement being activated via CCP and ACP, because the treatment of EA and Y with C3-depleted carp serum produced no effect. It was also found that the treatment of EAC and YC with the Fab fragment of anti-carp C3 (rabbit IgG) abolished the opsonising effect of carp complement deposited on the target cells. These results indicate that C3 is the major phagocytosis-promoting factor (opsonin). In addition, it was also demonstrated that pretreatment of the neutrophil surface with trypsin greatly decreased the phagocytosis of EAC, suggesting the presence of C3 receptors on the neutrophil surface.
AB - The opsonic effect of carp complement on the phagocytosis by carp peritoneal neutrophils was investigated using antibody-sensitised sheep erythrocytes (EA) and baker's yeast (Y), which are the activators of the classical complement pathway (CCP) and the alternative complement pathway (ACP), respectively. Carp antibody (A) exerted little opsonic effect on the phagocytosis of sheep erythrocytes (E). The treatment of EA and Y with normal carp serum (C) greatly enhanced phagocytosis. Such opsonic effect is apparently due to the complement being activated via CCP and ACP, because the treatment of EA and Y with C3-depleted carp serum produced no effect. It was also found that the treatment of EAC and YC with the Fab fragment of anti-carp C3 (rabbit IgG) abolished the opsonising effect of carp complement deposited on the target cells. These results indicate that C3 is the major phagocytosis-promoting factor (opsonin). In addition, it was also demonstrated that pretreatment of the neutrophil surface with trypsin greatly decreased the phagocytosis of EAC, suggesting the presence of C3 receptors on the neutrophil surface.
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U2 - 10.1016/S1050-4648(06)80028-8
DO - 10.1016/S1050-4648(06)80028-8
M3 - Article
AN - SCOPUS:0001827647
SN - 1050-4648
VL - 2
SP - 69
EP - 78
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 1
ER -