TY - JOUR
T1 - On the mechanism of cell lysis by deformation
AU - Takamatsu, Hiroshi
AU - Takeya, Ryu
AU - Naito, Seiji
AU - Sumimoto, Hideki
N1 - Funding Information:
This study was partly supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (Nos. 11650227 and 15360115). The authors thank Ms. Nichika Kouno and Ms. Yuko Fukuda for their contribution to the experiments.
PY - 2005/1
Y1 - 2005/1
N2 - In this study, we identify the extent of deformation that causes cell lysis using a simple technique where a drop of cell suspension is compressed by two flat plates. The viability of human prostatic adenocarcinoma PC-3 cells in solutions of various concentrations of NaCl is determined as a function of the gap size between the plates. The viability declines with decreasing gap size in the following order: 700mM > 150mM > 75mM NaCl. This is considered to be due to the difference in cell size, which is caused by the osmotic volume change before deformation; cell diameter becomes smaller in a solution of higher NaCl concentration, which appears to increase the survival ratio in a given gap size. The deformation-induced decrease in cell viability is correlated with the cell surface strain, which is dependent on the increase in surface area, irrespective of NaCl concentration. In addition, the treatment of cells with cytochalasin D results in the disappearance of cortical actin filaments and a marked drop in the viability, indicating that cell lysis is closely related to the deformation of the cytoskeleton.
AB - In this study, we identify the extent of deformation that causes cell lysis using a simple technique where a drop of cell suspension is compressed by two flat plates. The viability of human prostatic adenocarcinoma PC-3 cells in solutions of various concentrations of NaCl is determined as a function of the gap size between the plates. The viability declines with decreasing gap size in the following order: 700mM > 150mM > 75mM NaCl. This is considered to be due to the difference in cell size, which is caused by the osmotic volume change before deformation; cell diameter becomes smaller in a solution of higher NaCl concentration, which appears to increase the survival ratio in a given gap size. The deformation-induced decrease in cell viability is correlated with the cell surface strain, which is dependent on the increase in surface area, irrespective of NaCl concentration. In addition, the treatment of cells with cytochalasin D results in the disappearance of cortical actin filaments and a marked drop in the viability, indicating that cell lysis is closely related to the deformation of the cytoskeleton.
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U2 - 10.1016/j.jbiomech.2004.03.011
DO - 10.1016/j.jbiomech.2004.03.011
M3 - Article
C2 - 15519346
AN - SCOPUS:7044268943
SN - 0021-9290
VL - 38
SP - 117
EP - 124
JO - Journal of Biomechanics
JF - Journal of Biomechanics
IS - 1
ER -