TY - JOUR
T1 - Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA–RNA interface between two structural modules
AU - Tsuruga, Ryusei
AU - Uehara, Narumi
AU - Suzuki, Yuki
AU - Furuta, Hiroyuki
AU - Sugiyama, Hiroshi
AU - Endo, Masayuki
AU - Matsumura, Shigeyoshi
AU - Ikawa, Yoshiya
N1 - Funding Information:
This work was supported by MEXT KAKENHI Grant Number JP15K05561 (Y.I.). This work was also supported partly by University of Toyama Discretionary Funds of the President “Toyama RNA Research Alliance” (to Y.I. and S.M.).
Funding Information:
This work was supported by MEXT KAKENHI Grant Number JP15K05561 (Y.I.). This work was also supported partly by University of Toyama Discretionary Funds of the President ?Toyama RNA Research Alliance? (to Y.I. and S.M.).
PY - 2019/10
Y1 - 2019/10
N2 - Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules.
AB - Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules.
UR - http://www.scopus.com/inward/record.url?scp=85065800146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065800146&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2019.04.003
DO - 10.1016/j.jbiosc.2019.04.003
M3 - Article
C2 - 31109874
AN - SCOPUS:85065800146
SN - 1389-1723
VL - 128
SP - 410
EP - 415
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 4
ER -