Aim: To evaluate ocular surface molecules after transconjunctival pars plana vitrectomy (PPV). Methods: A total of 28 eyes of 28 patients who received PPV were examined. Tears (10 μl) were collected before and after 20-gauge PPV or 23-gauge PPV. The concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70 and tumour necrosis factor (TNF)-α were measured. The concentrations of each cytokine before and after surgery were compared. The change ratios of each cytokine (post-/presurgery) between 20-gauge and 23-gauge PPV were compared. Results: IL-1β, IL-6, IL-8 increased after PPV (Wilcoxon rank sum test; IL-1 β, p=0.009; IL-6, p<0.001; IL-8, p<0.001), while the other cytokines did not. Among them, only IL-8 increased significantly in 20-gauge PPV than in 23-gauge PPV (p=0.01, Mann-Whitney U test). Multivariate analysis showed that 20-gauge PPV was a significant factor behind the increase in postoperative IL-8 (OR 5.21, 95% CI (1.46 to 18.55), p=0.018), but surgical time and simultaneous cataract surgery were not significant factors. Conclusions: The increase in inflammatory cytokine IL-8 in tear was significantly less after 23-gauge PPV than 20-gauge PPV, indicating that transconjunctival PPV is less invasive from the viewpoint of the ocular surface molecular biology. This information may be important when deciding the method of PPV.
All Science Journal Classification (ASJC) codes
- Sensory Systems
- Cellular and Molecular Neuroscience