TY - JOUR
T1 - Occurrence of sul and tet(M) genes in bacterial community in Japanese marine aquaculture environment throughout the year
T2 - Profile comparison with Taiwanese and Finnish aquaculture waters
AU - Suzuki, Satoru
AU - Nakanishi, Sayoko
AU - Tamminen, Manu
AU - Yokokawa, Taichi
AU - Sato-Takabe, Yuki
AU - Ohta, Kohei
AU - Chou, Hsin Yiu
AU - Muziasari, Windi I.
AU - Virta, Marko
N1 - Funding Information:
This study was supported by Grants from KAKENHI (25257402, 22241014) from JSPS, Japan and JSPS-AF Joint Project, Maj and Tor Nessling Foundation and Academy of Finland. We thank to Mrs. M. Ogo, A. Kadoya, Mr. S. Nishioka and Dr. A. Karkman for their technical assistance.
Funding Information:
This study was supported by Grants from KAKENHI ( 25257402 , 22241014 ) from JSPS, Japan and JSPS-AF Joint Project, Maj and Tor Nessling Foundation and Academy of Finland. We thank to Mrs. M. Ogo, A. Kadoya, Mr. S. Nishioka and Dr. A. Karkman for their technical assistance.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/6/15
Y1 - 2019/6/15
N2 - The use of antibiotics in aquaculture causes selection pressure for antibiotic-resistant bacteria (ARB). Antibiotic resistance genes (ARGs) may persist in ARB and the environment for long time even after stopping drug administration. Here we show monthly differences in the occurrences of genes conferring resistance to sulfonamides (i.e. sul1, sul2, sul3), and tetracyclines (tet(M)) in Japanese aquaculture seawater accompanied by records of drug administration. sul2 was found to persist throughout the year, whereas the occurrences of sul1, sul3, and tet(M) changed month-to-month. sul3 and tet(M) were detected in natural bacterial assemblages in May and July, but not in colony-forming bacteria, thus suggesting that the sul3 was harbored by the non-culturable fraction of the bacterial community. Comparison of results from Taiwanese, Japanese, and Finnish aquaculture waters reveals that the profile of sul genes and tet(M) in Taiwan resembles that in Japan, but is distinct from that in Finland. To our knowledge, this work represents the first report to use the same method to compare the dynamics of sul genes and tet(M) in aquaculture seawater in different countries.
AB - The use of antibiotics in aquaculture causes selection pressure for antibiotic-resistant bacteria (ARB). Antibiotic resistance genes (ARGs) may persist in ARB and the environment for long time even after stopping drug administration. Here we show monthly differences in the occurrences of genes conferring resistance to sulfonamides (i.e. sul1, sul2, sul3), and tetracyclines (tet(M)) in Japanese aquaculture seawater accompanied by records of drug administration. sul2 was found to persist throughout the year, whereas the occurrences of sul1, sul3, and tet(M) changed month-to-month. sul3 and tet(M) were detected in natural bacterial assemblages in May and July, but not in colony-forming bacteria, thus suggesting that the sul3 was harbored by the non-culturable fraction of the bacterial community. Comparison of results from Taiwanese, Japanese, and Finnish aquaculture waters reveals that the profile of sul genes and tet(M) in Taiwan resembles that in Japan, but is distinct from that in Finland. To our knowledge, this work represents the first report to use the same method to compare the dynamics of sul genes and tet(M) in aquaculture seawater in different countries.
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U2 - 10.1016/j.scitotenv.2019.03.111
DO - 10.1016/j.scitotenv.2019.03.111
M3 - Article
C2 - 30889452
AN - SCOPUS:85062895688
SN - 0048-9697
VL - 669
SP - 649
EP - 656
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -
