Observation of glycolytic metabolites in tumor cell lysate by using hyperpolarization of deuterated glucose

Keiko Kumagai; Mai Akakabe; Masayuki Tsuda; Masashi Tsuda; Eri Fukushi; Jun Kawabata; Takamasa Abe; Kazuhiro Ichikawa

doi:10.1248/bpb.b14-00156

Observation of glycolytic metabolites in tumor cell lysate by using hyperpolarization of deuterated glucose

Keiko Kumagai, Mai Akakabe, Masayuki Tsuda, Masashi Tsuda, Eri Fukushi, Jun Kawabata, Takamasa Abe, Kazuhiro Ichikawa

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of ¹³C₆-glucose-d₇ and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin-lattice relaxation time T₁. ¹³C₆-Glucose-d₇ exhibited a T₁ that was over ten times as long as that of ¹³C₆-glucose, and metabolic NMR studies of hyperpolarized ¹³C₆-glucose-d₇ using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.

Original language	English
Pages (from-to)	1416-1421
Number of pages	6
Journal	Biological and Pharmaceutical Bulletin
Volume	37
Issue number	8
DOIs	https://doi.org/10.1248/bpb.b14-00156
Publication status	Published - Aug 1 2014

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmaceutical Science

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abstract = "Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of 13C6-glucose-d7 and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin-lattice relaxation time T1. 13C6-Glucose-d7 exhibited a T1 that was over ten times as long as that of 13C6-glucose, and metabolic NMR studies of hyperpolarized 13C6-glucose-d7 using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.",

author = "Keiko Kumagai and Mai Akakabe and Masayuki Tsuda and Masashi Tsuda and Eri Fukushi and Jun Kawabata and Takamasa Abe and Kazuhiro Ichikawa",

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AU - Kumagai, Keiko

AU - Akakabe, Mai

AU - Tsuda, Masayuki

AU - Tsuda, Masashi

AU - Fukushi, Eri

AU - Kawabata, Jun

AU - Abe, Takamasa

AU - Ichikawa, Kazuhiro

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AB - Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of 13C6-glucose-d7 and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin-lattice relaxation time T1. 13C6-Glucose-d7 exhibited a T1 that was over ten times as long as that of 13C6-glucose, and metabolic NMR studies of hyperpolarized 13C6-glucose-d7 using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.

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Observation of glycolytic metabolites in tumor cell lysate by using hyperpolarization of deuterated glucose

Abstract

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