Nuclease tolerant FRET probe based on DNA-quantum dot conjugation

Daisuke Onoshima; Noritada Kaji; Manabu Tokeshi; Yoshinobu Baba

doi:10.2116/analsci.24.181

Nuclease tolerant FRET probe based on DNA-quantum dot conjugation

Daisuke Onoshima, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI). 2008

Original language	English
Pages (from-to)	181-183
Number of pages	3
Journal	analytical sciences
Volume	24
Issue number	2
DOIs	https://doi.org/10.2116/analsci.24.181
Publication status	Published - Feb 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.2116/analsci.24.181

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abstract = "We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI). 2008",

author = "Daisuke Onoshima and Noritada Kaji and Manabu Tokeshi and Yoshinobu Baba",

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AU - Kaji, Noritada

AU - Tokeshi, Manabu

AU - Baba, Yoshinobu

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AB - We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI). 2008

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Nuclease tolerant FRET probe based on DNA-quantum dot conjugation

Abstract

All Science Journal Classification (ASJC) codes

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