TY - JOUR
T1 - Nuclear translocation of the Y-box binding protein by ultraviolet irradiation
AU - Koike, Koji
AU - Uchiumi, Takeshi
AU - Ohga, Takefumi
AU - Toh, Satoshi
AU - Wada, Morimasa
AU - Kohno, Kimitoshi
AU - Kuwano, Michihiko
N1 - Funding Information:
We thank Takanori Nakamura in our laboratory for fruitful discussion. This study was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture of Japan, and by the Fukuoka Anticancer Research Fund.
PY - 1997/11/17
Y1 - 1997/11/17
N2 - The Y-box binding protein, YB-1, is a member of a DNA binding protein family with a structurally and functionally conserved cold shock domain. Using Western blotting and immunohistochemical methods, larger amounts of YB-1 were detected in the cytosol, particularly at the perinuclear region, than in the nucleus of human cancer cells. UV irradiation increased accumulation of YB-1 in the nucleus at 20 min and thereafter. This translocation of YB-1 into the nucleus by UV irradiation was blocked by the protein kinase inhibitor H-7, but not HA-1004. Both green fluorescent protein (GFP)-YB-1 and GFP-YB-1C with the C-terminus (248-317) of YB-1 were located mainly in the cytosol, but GFP-YB-1ΔC with a deletion at the C-terminus of YB-1 was located in the nucleus. YB-1 is translocated into the nucleus by UV irradiation, possibly through a protein kinase C-mediated signal transduction pathway, and the C-terminal region of YB-1 might be important for cytoplasmic retention of YB-1.
AB - The Y-box binding protein, YB-1, is a member of a DNA binding protein family with a structurally and functionally conserved cold shock domain. Using Western blotting and immunohistochemical methods, larger amounts of YB-1 were detected in the cytosol, particularly at the perinuclear region, than in the nucleus of human cancer cells. UV irradiation increased accumulation of YB-1 in the nucleus at 20 min and thereafter. This translocation of YB-1 into the nucleus by UV irradiation was blocked by the protein kinase inhibitor H-7, but not HA-1004. Both green fluorescent protein (GFP)-YB-1 and GFP-YB-1C with the C-terminus (248-317) of YB-1 were located mainly in the cytosol, but GFP-YB-1ΔC with a deletion at the C-terminus of YB-1 was located in the nucleus. YB-1 is translocated into the nucleus by UV irradiation, possibly through a protein kinase C-mediated signal transduction pathway, and the C-terminal region of YB-1 might be important for cytoplasmic retention of YB-1.
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U2 - 10.1016/S0014-5793(97)01296-9
DO - 10.1016/S0014-5793(97)01296-9
M3 - Article
C2 - 9409758
AN - SCOPUS:0030825963
SN - 0014-5793
VL - 417
SP - 390
EP - 394
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -
