TY - JOUR
T1 - Nuclear export signal in CDC25B
AU - Uchida, Sanae
AU - Ohtsubo, Motoaki
AU - Shimura, Mari
AU - Hirata, Masato
AU - Nakagama, Hitoshi
AU - Matsunaga, Tsukasa
AU - Yoshida, Minoru
AU - Ishizaka, Yukihito
AU - Yamashita, Katsumi
N1 - Funding Information:
We thank H. Okayama (University of Tokyo) and A. Miyawaki (RIKEN) for the generous gifts of CDC25B1 cDNA and Venus, a modified GFP expression plasmid, respectively. This work was supported in part by Grants-in-Aid for Scientific Research (to K.Y. and Y.I.) and for the Second Term of the Comprehensive 10-Year Strategy for Cancer Control (to H.N.) from the Ministry of Health, Labor, and Welfare and by Grants-in-Aid of Scientific Research from the Japan Society for the Promotion of Science, the Ministry of Education, Science, Sports and Culture of Japan (to M.H. and T.M.).
PY - 2004/3/26
Y1 - 2004/3/26
N2 - CDC25B is a dual-specificity phosphatase that activates CDK1/cyclin B. The nuclear exclusion of CDC25B is controlled by the binding of 14-3-3 to the nuclear export signal (NES) of CDC25B, which was reported to be amino acids H28 to L40 in the N-terminal region of CDC25B. In studying the subcellular localization of CDC25B, we found a functional NES at V52 to L65, the sequence of which is VTTLTQTMHDLAGL, where bold letters are leucine or hydrophobic amino acids frequently seen in an NES. The deletion of this NES sequence caused the mutant protein to locate exclusively in nuclei, while NES-fused GFP was detected in the cytoplasm. Moreover, the introduction of point mutations at some of the critical amino acids impaired cytoplasmic localization. Treatment with leptomycin B, a potent inhibitor of CRM1/exportin1, disrupted the cytoplasmic localization of both Flag-tagged CDC25B and NES-fused GFP. From these results, we concluded that the sequence we found is a bona fide NES of CDC25B.
AB - CDC25B is a dual-specificity phosphatase that activates CDK1/cyclin B. The nuclear exclusion of CDC25B is controlled by the binding of 14-3-3 to the nuclear export signal (NES) of CDC25B, which was reported to be amino acids H28 to L40 in the N-terminal region of CDC25B. In studying the subcellular localization of CDC25B, we found a functional NES at V52 to L65, the sequence of which is VTTLTQTMHDLAGL, where bold letters are leucine or hydrophobic amino acids frequently seen in an NES. The deletion of this NES sequence caused the mutant protein to locate exclusively in nuclei, while NES-fused GFP was detected in the cytoplasm. Moreover, the introduction of point mutations at some of the critical amino acids impaired cytoplasmic localization. Treatment with leptomycin B, a potent inhibitor of CRM1/exportin1, disrupted the cytoplasmic localization of both Flag-tagged CDC25B and NES-fused GFP. From these results, we concluded that the sequence we found is a bona fide NES of CDC25B.
UR - http://www.scopus.com/inward/record.url?scp=1542375440&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1542375440&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.02.039
DO - 10.1016/j.bbrc.2004.02.039
M3 - Article
C2 - 15003534
AN - SCOPUS:1542375440
SN - 0006-291X
VL - 316
SP - 226
EP - 232
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -