Novel Leu-Lys-specific peptidase (leulysin) produced by gel-entrapped yeast cells

Tsutomu Okada, Kenji Sonomoto, Atsuo Tanaka

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Saccharomyces cerevisiae cells entrapped with a neutral hydrophilic photo-crosslinkable resin prepolymers specifically excreted into a cultured medium a new type of a peptidase, which cleaved Leu-Lys bond of α-mating factor. The enzyme was purified by membrane filtration followed by ion exchange chromatography and gel filtration chromatography. The purified enzyme showed a strict substrate specificity on internal Leu-Lys bond. Leu-Lys bond near terminus of a molecule and Leu-X and X-Lys bonds examined so far were not hydrolyzed by the enzyme.

Original languageEnglish
Pages (from-to)316-322
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - May 29 1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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