Novel DNA probes capable of discriminating Indica and Japonica rice cultivars

Shinya Oba; Mikio Hanzawa; Toyoaki Anai; Masaaki Umeda; Hirofumi Uchimiya

doi:10.3109/10425179609020878

Novel DNA probes capable of discriminating Indica and Japonica rice cultivars

Shinya Oba, Mikio Hanzawa, Toyoaki Anai, Masaaki Umeda, Hirofumi Uchimiya

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We obtained two DNA clones, G93 (1.6kb) and G318 (1.7kb) from indica rice (Oryza sativa L.) cultivar Culture 340. Southern hybridization analysis indicated that both DNA probes hybridized with DNA of indica cultivars but not with japonica cultivars. Some javanica cultivars DNAs did not hybridize with these probes. Nucleotide sequence (400bp) of a part of G93 showed a 65.8% G + C content. These novel DNA probes can be used for the study of evolutional fingerprinting of rice cultivars.

Original language	English
Pages (from-to)	303-306
Number of pages	4
Journal	Mitochondrial DNA
Volume	6
Issue number	5
DOIs	https://doi.org/10.3109/10425179609020878
Publication status	Published - 1996
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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title = "Novel DNA probes capable of discriminating Indica and Japonica rice cultivars",

abstract = "We obtained two DNA clones, G93 (1.6kb) and G318 (1.7kb) from indica rice (Oryza sativa L.) cultivar Culture 340. Southern hybridization analysis indicated that both DNA probes hybridized with DNA of indica cultivars but not with japonica cultivars. Some javanica cultivars DNAs did not hybridize with these probes. Nucleotide sequence (400bp) of a part of G93 showed a 65.8% G + C content. These novel DNA probes can be used for the study of evolutional fingerprinting of rice cultivars.",

author = "Shinya Oba and Mikio Hanzawa and Toyoaki Anai and Masaaki Umeda and Hirofumi Uchimiya",

note = "Funding Information: We thank Dr. k. Kikuchi and Dr. H. Sano for the rice cultivars S 0 was the recipient of a JSPS fellowship for japines(~J unior Scientists. This research was supported in part by Grants-in-Aid for Science Research from the Ministry of Educatiion, Science and Culture of lapan, ,ind grant from the Rockefeller Foundation.",

year = "1996",

doi = "10.3109/10425179609020878",

language = "English",

volume = "6",

pages = "303--306",

journal = "Mitochondrial DNA",

issn = "1940-1736",

publisher = "Informa Healthcare",

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T1 - Novel DNA probes capable of discriminating Indica and Japonica rice cultivars

AU - Oba, Shinya

AU - Hanzawa, Mikio

AU - Anai, Toyoaki

AU - Umeda, Masaaki

AU - Uchimiya, Hirofumi

N1 - Funding Information: We thank Dr. k. Kikuchi and Dr. H. Sano for the rice cultivars S 0 was the recipient of a JSPS fellowship for japines(~J unior Scientists. This research was supported in part by Grants-in-Aid for Science Research from the Ministry of Educatiion, Science and Culture of lapan, ,ind grant from the Rockefeller Foundation.

PY - 1996

Y1 - 1996

N2 - We obtained two DNA clones, G93 (1.6kb) and G318 (1.7kb) from indica rice (Oryza sativa L.) cultivar Culture 340. Southern hybridization analysis indicated that both DNA probes hybridized with DNA of indica cultivars but not with japonica cultivars. Some javanica cultivars DNAs did not hybridize with these probes. Nucleotide sequence (400bp) of a part of G93 showed a 65.8% G + C content. These novel DNA probes can be used for the study of evolutional fingerprinting of rice cultivars.

AB - We obtained two DNA clones, G93 (1.6kb) and G318 (1.7kb) from indica rice (Oryza sativa L.) cultivar Culture 340. Southern hybridization analysis indicated that both DNA probes hybridized with DNA of indica cultivars but not with japonica cultivars. Some javanica cultivars DNAs did not hybridize with these probes. Nucleotide sequence (400bp) of a part of G93 showed a 65.8% G + C content. These novel DNA probes can be used for the study of evolutional fingerprinting of rice cultivars.

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JO - Mitochondrial DNA

JF - Mitochondrial DNA

IS - 5

ER -

Novel DNA probes capable of discriminating Indica and Japonica rice cultivars

Abstract

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