TY - GEN
T1 - Noninvasive sensing of skin cancer for early diagnosis using cathespin L
AU - Yamaguchi, Masaki
AU - Date, Akira
AU - Suzuki, Wataru
AU - Tahara, Yusuke
AU - Sasaki, Makoto
PY - 2010
Y1 - 2010
N2 - It is reported that Cathepsin L (CatL) activity is correlated with actinic keratosis and squamous cell cancers. The purpose of this study is to propose an on-site testing of CatL activity in the skin, which can be used for the diagnosis of skin conditions noninvasively, easily, quickly and at any time. In this study, a novel immunochromatographic test-strip is proposed for CatL analysis in the skin. In order to realize the disposable test-strip, a colloidal gold technique was selected as the molecular recognition method for the CatL. A human skin sample was collected using tape stripping. The fabricated immuno-chromatographic test-strip (test-strip) consists of four pads, a sample pad, a conjugate release pad, a nitrocellulose membrane and an absorption pad. Based on optimal assay conditions, the sensitivity of the test-strip was measured. It required 90 s for a sample to reach the test-line on the test-strip. The optical density was proportion to the activity of CatL. In order to cancel out the influence of the collecting technique, the CatL/TP ratio was evaluated. It was suggested that the test-strip might be used for the qualitative analysis of CatL in the stratum corneum.
AB - It is reported that Cathepsin L (CatL) activity is correlated with actinic keratosis and squamous cell cancers. The purpose of this study is to propose an on-site testing of CatL activity in the skin, which can be used for the diagnosis of skin conditions noninvasively, easily, quickly and at any time. In this study, a novel immunochromatographic test-strip is proposed for CatL analysis in the skin. In order to realize the disposable test-strip, a colloidal gold technique was selected as the molecular recognition method for the CatL. A human skin sample was collected using tape stripping. The fabricated immuno-chromatographic test-strip (test-strip) consists of four pads, a sample pad, a conjugate release pad, a nitrocellulose membrane and an absorption pad. Based on optimal assay conditions, the sensitivity of the test-strip was measured. It required 90 s for a sample to reach the test-line on the test-strip. The optical density was proportion to the activity of CatL. In order to cancel out the influence of the collecting technique, the CatL/TP ratio was evaluated. It was suggested that the test-strip might be used for the qualitative analysis of CatL in the stratum corneum.
UR - http://www.scopus.com/inward/record.url?scp=79953808147&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953808147&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:79953808147
SN - 9780889868274
T3 - Proceedings of the 7th IASTED International Conference on Biomedical Engineering, BioMED 2010
SP - 43
EP - 47
BT - Proceedings of the 7th IASTED International Conference on Biomedical Engineering, BioMED 2010
T2 - 7th IASTED International Conference on Biomedical Engineering, BioMED 2010
Y2 - 17 February 2010 through 19 February 2010
ER -
