TY - JOUR
T1 - NMR-based conformational analysis of sphingomyelin in bicelles
AU - Yamaguchi, Toshiyuki
AU - Suzuki, Takashi
AU - Yasuda, Tomokazu
AU - Oishi, Tohru
AU - Matsumori, Nobuaki
AU - Murata, Michio
N1 - Funding Information:
We are grateful to Dr. Yuichi Umegawa and Mr. Mototsugu Doi, Osaka University, for their help in NMR measurements. This work was supported by Grant-In-Aids for JSPS Fellows, for Scientific Research (B) (No. 20310132) and (S) (No. 18101010), and by SUNBOR grant from Suntory Institute for Bioorganic Research, Japan. T.Y. expresses special thanks for the Global Center of Excellence (COE) Program ‘Global Education and Research Center for Bio-Environmental Chemistry’ of Osaka University.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Sphingomyelin (SM) is a common sphingolipid in mammalian membranes and is known to be substantially involved in cellular events such as the formation of lipid rafts. Despite its biological significance, conformation of SM in a membrane environment remains unclear because the noncrystalline property and anisotropic environment of lipid bilayers hampers the application of X-ray crystallography and NMR measurements. In this study, to elucidate the conformation of SM in membranes, we utilized bicelles as a substitute for a lipid bilayer membrane. First, we demonstrated through 31P NMR, 2H NMR, and dynamic light scattering experiments that SM forms both oriented and isotropic bicelles by changing the ratio of SM/dihexanoyl phosphatidylcholine. Then, we determined the conformation of SM in isotropic bicelles on the basis of coupling constants and NOE correlations in 1H NMR and found that the C2-C6 and amide groups of SM take a relatively rigid conformation in bicelles.
AB - Sphingomyelin (SM) is a common sphingolipid in mammalian membranes and is known to be substantially involved in cellular events such as the formation of lipid rafts. Despite its biological significance, conformation of SM in a membrane environment remains unclear because the noncrystalline property and anisotropic environment of lipid bilayers hampers the application of X-ray crystallography and NMR measurements. In this study, to elucidate the conformation of SM in membranes, we utilized bicelles as a substitute for a lipid bilayer membrane. First, we demonstrated through 31P NMR, 2H NMR, and dynamic light scattering experiments that SM forms both oriented and isotropic bicelles by changing the ratio of SM/dihexanoyl phosphatidylcholine. Then, we determined the conformation of SM in isotropic bicelles on the basis of coupling constants and NOE correlations in 1H NMR and found that the C2-C6 and amide groups of SM take a relatively rigid conformation in bicelles.
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U2 - 10.1016/j.bmc.2011.11.001
DO - 10.1016/j.bmc.2011.11.001
M3 - Article
C2 - 22133901
AN - SCOPUS:84855204980
SN - 0968-0896
VL - 20
SP - 270
EP - 278
JO - Bioorganic and Medicinal Chemistry
JF - Bioorganic and Medicinal Chemistry
IS - 1
ER -