NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis

Dawei Yan, Vanathy Easwaran, Vivian Chau, Masanori Okamoto, Matthew Ierullo, Mitsuhiro Kimura, Akira Endo, Ryoichi Yano, Asher Pasha, Yunchen Gong, Yong Mei Bi, Nicolas Provart, David Guttman, Anne Krapp, Steven J. Rothstein, Eiji Nambara

Research output: Contribution to journalArticlepeer-review

132 Citations (Scopus)

Abstract

Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling.

Original languageEnglish
Article number13179
JournalNature communications
Volume7
DOIs
Publication statusPublished - Oct 12 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology
  • General Physics and Astronomy

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